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Zymoclean™ Gel RNA Recovery Kit

Quick (30 minute) recovery of purified RNA fragments from agarose gels.
Fast-Spin column technology allows RNA to be eluted into minimal volumes (≥ 6 µl).
Recovery ≥ 80% for RNA > 500 nt.

Product Size Catalog # Price Qty
Zymoclean™ Gel RNA Recovery Kit 50 Preps. R1011

About Zymoclean™ Gel RNA Recovery Kit

The Zymoclean™ Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Fast-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).

Format Spin Column
Processing Time 30 min
Sample Sources Single- or double-stranded RNA fragments (≥200 nucleotides) resolved in MOPS, TAE and TBE buffered agarose gels.
RNA Purity High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations.
RNA Recovery The recovery rate for fragments ≥500 nucleotides is ≥80 %. Total binding capacity of the supplied Zymo-Spin IC™ Columns is ≤ 5 µg.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.
Equipment Needed Microcentrifuge, 37 to 65ºC heat source.

The recovery of RNA from an agarose gel. Different sized RNAs on the left were excised from the gel and recovered using the Zymoclean™ Gel RNA Recovery Kit (lanes 1-4)

Step 1

Excise the RNA fragment from the agarose1 gel using a razor blade or scalpel and transfer it to a 1.5 ml microcentrifuge tube.

Step 2

Add 3 volumes of RAD Buffer™ to each volume of agarose excised from the gel.

Step 3

Incubate2 at 37-55°C for 5-10 minutes or until the gel slice is completely dissolved.

Step 4

Transfer the mixture to Zymo-Spin™ IC Column in a Collection Tube.

Step 5

Centrifuge at ≥12,000 x g for 2 minutes.  Discard the flow-through.

Step 6

Add 400 µl RNA Prep Buffer to the column.  Centrifuge at ≥12,000 x g for 1 minute.  Discard the flow-through and replace the Zymo-Spin™ IC Column back into the Collection Tube.

Step 7

Add 800 µl RNA Wash Buffer to the column.  Centrifuge at ≥12,000 x g for 30 seconds.  Discard the flow-through and replace the Zymo-Spin™ IC Column back into the Collection Tube.  Repeat the wash step with 400 µl RNA Wash Buffer

Step 8

Centrifuge the Zymo-Spin™ IC Column at ≥12,000 x g for 2 minutes in the emptied Collection Tube to ensure complete removal of the wash buffer.

Step 9

Carefully remove the Zymo-Spin™ IC Column from the Collection Tube and place into a DNase/RNase-Free Tube.  Add ≥6 µl DNase/RNase-Free Water3 directly to the column matrix and let stand for 1 minute.

Step 10

Centrifuge at 10,000 × g for 30 seconds to elute the RNA from the column.  RNA can be used immediately or stored at ≤-70 oC (see Specifications, page 1).

For specific notes and additional information, please see the product protocol PDF.
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“I did a direct comparison with the Qiagen kit (which we normally use) on duplicate samples. I found the yields to be the same, but the high concentration from Zymo resulting from the low elution volume was extremely helpful for a downstream ligation. That ligation also showed that the DNA products from both kits performed exactly the same (within the resolution of a standard gel). The elution concentration is probably the best aspect. The reduced number of buffers and steps is also nice, as are the low-bind column containers. I'll probably be using this kit from now on.”
Adam C. (University of Texas, Southwestern)
“I had tried three other kits to do a certain recovery before this, I was getting bad yields, the elution volume required was quite large, the protocols were not as easy to follow and they took longer to complete. I liked everything about this kit, it is simple, efficient and quick. The protocol is easy to follow and the yield was 4-5 times better than what I got from other kits.”
Wendy P. (University of Auckland)
“It's a kit with a faster protocol and [has] a very good efficiency in quantity of DNA recovered. Also have a possibility to elute only in 6 µl of water. “
Joao B, (Instituto de Tecnologia Química e Biológica)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
Simin H. (University of California, Irvine)
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
Thomas B (University of Tennessee, Knoxville)
“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”
Kelly-Anne F.
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
Ingrid M.
Click here to submit your review of the Zymoclean™ Gel RNA Recovery Kit.

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