About Quick-RNA Fungal/Bacterial Microprep™
Note: The Quick-RNA Fungal/Bacterial Microprep™ (R2010) has been updated to Quick-RNA™ Fungal/Bacterial Microprep; all components and protocols are the same. Catalog numbers and names of components may have been modified but original buffer recipe remains the same.
The Quick-RNA™ Fungal/Bacterial MicroPrep provides for rapid isolation of RNA from pelleted tough-to-lyse bacterial (e.g., Gram-positive), yeast or fungal cells. The Quick-RNA™ Fungal/Bacterial MicroPrep employs ultra-high density ZR BashingBeads™ for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples.
The Zymo-Spin™ IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IC Column efficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.
|Format||Bead beating, spin column.|
|Equipment||Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional).|
|Sample Sources||10-20 mg (wet weight) fungi or bacteria. This equates to approximately 2x108 bacterial cells and 2x107 yeast cells.|
|RNA Purity||High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.|
|RNA Recovery||RNA can be eluted into small volumes, ≥6 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~10 µg.|
|Compatibility||Compatible with samples stored in RNAlater™.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.|
Total RNA was isolated from equal amounts of pelleted E. coli cells containing plasmid DNA (pGEM®) using the Quick-RNA™ Fungal/Bacterial MicroPrep or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used.
* = genomic (< 10 kb) and plasmid (< 3 kb) DNA contamination;
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