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Quick-RNA Fungal/Bacterial Microprep™

Quick, 10 minute isolation of total RNA (~10 µg) from Gram-negative/positive bacteria, yeast and fungi using ultra-high density BashingBeads™ and Zymo-Spin™ column technologies.
High-quality RNA eluted in ≥6 µl is ready for reverse transcription, microarray, sequencing, etc.

Product Size Catalog # Price Qty
Quick-RNA™ Fungal/Bacterial RNA Microprep 50 Preps. R2010

About Quick-RNA Fungal/Bacterial Microprep™

Note: The ZR Fungal/Bacterial RNA MicroPrep™ (R2010) has been updated to Quick-RNA™ Fungal/Bacterial Microprep; all components and protocols are the same. Catalog numbers and names of components may have been modified but original buffer recipe remains the same.

The Quick-RNA Fungal/Bacterial MicroPrep provides for rapid isolation of RNA from pelleted tough-to-lyse bacterial (e.g., Gram-positive), yeast or fungal cells.  The Quick-RNA Fungal/Bacterial MicroPrep  employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples. 

The Zymo-Spin IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IC Column efficiently binds total RNA.  The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.

Format Bead beating, spin column.
Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional).
Sample Sources 10-20 mg (wet weight) fungi or bacteria. This equates to approximately 2x108 bacterial cells and 2x107 yeast cells.
RNA Purity High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.
RNA Recovery RNA can be eluted into small volumes, ≥6 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~10 µg.
Compatibility Compatible with samples stored in RNAlater™.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.

High quality total RNA is isolated from different microbial species including gram negative bacteria (E. coli, P. aeruginosa, S. enterica), gram positive bacteria (E. faecalis, L. monocytogenes, L. fermentum), and yeast (S. cerevisiae) using the Quick-RNA™ Fungal/Bacterial system (Agilent 2200 TapeStation).

Click Here to download the protocol
For specific notes and additional information, please see the product protocol PDF.
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