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YeaStar™ RNA Kit

Recovery of purified RNA from a wide range of fungus species using Fast-Spin column technology.
Omits the use of glass beads and organic denaturants.
Eluted RNA is suitable for use in RT-PCR or other RNA-based procedures.

Product Size Catalog # Price Qty
YeaStar™ RNA Kit 40 Preps. R1002

About YeaStar™ RNA Kit

The YeaStar™ RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Fast-Spin column technology.

Format Spin Column
Processing Time 30 min
Equipment Microcentrifuge
Sample Sources Fungi susceptible to yeast lytic enzyme lysis.
RNA Purity High quality RNA is recovered in the supplied RNase-free water.
RNA Recovery Typically, RNA is eluted into 60 µl RNase-free water. The provided columns have an RNA binding capacity of 25 µg.
Sample Size 1 to 1.5 ml liquid culture.

Step 1

Pellet 1-5 x107 cells (1-1.5 ml culture) by centrifugation at 500 x g for 2 minutes.  Carefully remove all of the supernatant.

Step 2

Add 80 µl of the YR Digestion Buffer and 5 µl of the Zymolyase to the cell pellet and resuspend the pellet completely by pipetting.  Incubate the suspension at 30-37°C for 40-60 minutes2.

Step 3

Add 160 µl of the YR Lysis Buffer and mix thoroughly by vortexing.

Step 4

Centrifuge the mixture at 7,000 x g for 2 minutes3.

Step 5

Transfer the supernatant to the Zymo-Spin™ IIIC Column in a Collection Tube and centrifuge at ³10,000 x g for 1 minute.

Step 6

Add 200 µl RNA Wash Buffer to the column and centrifuge at ³10,000 x g for 1 minute.  Discard the flow-through.  Repeat the wash step.

Step 7

Remove the Zymo-Spin IIIC Column carefully from the Collection Tube and transfer it into an RNase-Free Tube.  Add 60 µl of DNase/RNase-Free Water directly to the column matrix4.  Centrifuge at ≥10,000 x g for 30 seconds. The eluted RNA can be used immediately or stored at -70ºC.

For specific notes and additional information, please see the product protocol PDF.
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