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ZR Fungal/Bacterial RNA MiniPrep™

Quick, 10 minute isolation of total RNA (~50 µg) from Gram-negative/positive bacteria, yeast and fungi using ultra-high density BashingBeads™ and Zymo-Spin™ column technologies.
High-quality RNA eluted in ≥6 µl is ready for reverse transcription, microarray, sequencing, etc.

Product Size Catalog # Price Qty
ZR Fungal/Bacterial RNA MiniPrep™ 50 Preps R2014

About ZR Fungal/Bacterial RNA MiniPrep™

The ZR Fungal/Bacterial RNA MiniPrep provides for rapid isolation of RNA from pelleted tough-to-lyse bacterial (e.g., Gram-positive), yeast or fungal cells.  The ZR Fungal/Bacterial RNA MiniPrep employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples. 

The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently binds total RNA.  The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.

RNA can be eluted into volumes as little as 25 µl in less than 10 minutes.

Format Bead beating, spin column.
Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional).
Sample Sources 50-100 mg (wet weight) fungi or bacteria. This equates to approximately 109 bacterial cells and 108 yeast cells.
RNA Purity High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.
RNA Recovery RNA can be eluted into small volumes, ≥25 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~50 µg.
Compatibility Compatible with samples stored in RNAlater™.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.

Total RNA was isolated from equal amounts of pelleted E. coli cells containing plasmid DNA (pGEM®) using the ZR Fungal/Bacterial RNA MicroPrep™ or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used.

* = genomic (< 10 kb) and plasmid (< 3 kb) DNA contamination;
DNase I = samples treated with DNase I.

Click Here to download the protocol
For specific notes and additional information, please see the product protocol PDF.
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