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Quick-DNA/RNA™ Blood Tube Kit

For use with DNA/RNA Shield™ - Blood Collection Tube (R1150)
No reagent removal (no pelleting) – Direct sample processing.
Purify high quality DNA and/or total RNA (including small/micro RNAs) from the same whole blood sample. Ready for use in any downstream application. DNase I included.

Product Size Catalog # Price Qty
Quick-DNA/RNA™ Blood Tube Kit 50 Preps R1151

About Quick-DNA/RNA™ Blood Tube Kit

The Quick-DNA/RNA™ Blood Tube Kit is designed for use with the DNA/RNA Shield™ - Blood Collection Tube (R1150), enabling worry-free sample storage at ambient temperatures. The purification procedure uses Zymo-Spin™ column technology. Simply bind the sample onto the Zymo-Spin™ IIICG Column with the aid of reservoirs (vacuum compatible). There is no reagent removal and no pelleting for easy direct whole tube processing. High quality DNA and/or total RNA from 3 ml whole blood is eluted into ≥50 µl of DNase/RNase-free water and is ready for any downstream application including RTPCR, sequencing, etc.

High quality DNA and RNA is effectively purified from blood stored in DNA/RNA Shield™. High molecular weight DNA was intact with no apparent degradation. Also, RNA was high quality, DNA-free and included small RNAs. Linear recovery of DNA and RNA using the Quick-DNA/RNA™ Blood Tube Kit. Aliquots (1-3 ml) of whole blood stored in DNA/RNA Shield™ were used for purification (n=3)
RNA in blood is effectively stabilized in DNA/RNA Shield™ at ambient temperature. Graph shows cellular RNA from human whole blood stabilized in DNA/RNA Shield™ at the indicated time points and analyzed by (RT)qPCR Viruses, bacteria and yeast are effectively inactivated by DNA/RNA Shield™. Samples containing the infectious agent (virus, bacteria, yeast) were treated with DNA/RNA Shield™ or mock (PBS) treated for 5 minutes. CFU was separately quantified and titer (PFU) was subsequently determined by plaque assay.
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