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ZR Urine RNA Isolation Kit™

Quick, simple and reliable recovery of total RNA from cells, biological sediment in urine, large volume liquid samples and suitable for isolation of RNA from microvescicles.
Zymo-Spin™ column technology allows RNA to be eluted into volumes ≥6 µl and is ready for use in RT-PCR and other RNA-based procedures.

Product Size Catalog # Price Qty
ZR Urine RNA Isolation Kit™ 20 Preps. R1038
ZR Urine RNA Isolation Kit™ 50 Preps. R1039

About ZR Urine RNA Isolation Kit™

ZR Urine RNA Isolation Kit is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples.  The product enables isolation of cells from urine using a syringe (not provided) and a uniquely-designed syringe filter.  Following separation, cells are lysed and total RNA stabilized using a specially formulated Urine RNA Buffer.  The collected lysate can then be used immediately or at a later time following transportation and/or storage.  Also, the Urine RNA Buffer is ideal for direct isolation of RNA from microvesicles that may be recovered from urine filtrates. 

One-step RNA isolation occurs via matrix adsorption using Zymo-Spin IC Columns.  The RNA isolation procedure is simple and can be performed in less than 5 minutes.  Use of the ZR Urine RNA Isolation Kit™ results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.

Sample Sources Urine and other aqueous samples containing cells, biological sediment, microvesicle-associated RNA, etc.
RNA Purity High quality RNA is recovered in RNase-free water. Some DNA may be present in the final preparation of RNA. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.
RNA Recovery Typically, 0.2 to 3.0 µg RNA per 30 ml urine sample. The RNA binding capacity of the supplied columns is up to 10 µg.
Sample Size 30 ml (standard reaction); can be increased/decreased proportionally.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is recommended for prolonged storage.
Equipment Needed Microcentrifuge; syringes (i.e., 30 ml, 1 ml).

Click Here to download the protocol
For specific notes and additional information, please see the product protocol PDF.
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