About ZR Urine RNA Isolation Kit™
ZR Urine RNA Isolation Kit™ is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples. The product enables isolation of cells from urine using a syringe (not provided) and a uniquely-designed syringe filter. Following separation, cells are lysed and total RNA stabilized using a specially formulated Urine RNA Buffer. The collected lysate can then be used immediately or at a later time following transportation and/or storage. Also, the Urine RNA Buffer is ideal for direct isolation of RNA from microvesicles that may be recovered from urine filtrates.
One-step RNA isolation occurs via matrix adsorption using Zymo-Spin™ IC Columns. The RNA isolation procedure is simple and can be performed in less than 5 minutes. Use of the ZR Urine RNA Isolation Kit™ results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.
|Sample Sources||Urine and other aqueous samples containing cells, biological sediment, microvesicle-associated RNA, etc.|
|RNA Purity||High quality RNA is recovered in RNase-free water. Some DNA may be present in the final preparation of RNA. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.|
|RNA Recovery||Typically, 0.2 to 3.0 µg RNA per 30 ml urine sample. The RNA binding capacity of the supplied columns is up to 10 µg.|
|Sample Size||30 ml (standard reaction); can be increased/decreased proportionally.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is recommended for prolonged storage.|
|Equipment Needed||Microcentrifuge; syringes (i.e., 30 ml, 1 ml).|
|Click Here to download the protocol|