About Quick-RNA™ MicroPrep
The Quick-RNA™ MicroPrep kit is an innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Fast-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes.
The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the Zymo-Spin™ Columns. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for enrichment of small and large RNAs in two separate fractions.
|Sample Sources||Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.|
|Storage||Samples homogenized in RNA Lysis Buffer are stable and can be stored frozen prior to purification.|
|RNA Purity||High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations.|
|RNA Recovery||Up to 10 µg RNA can be eluted into ≥6 µl RNase-free water allowing for a highly concentrated sample.|
|Sample Size||Up to 106 cells or 5 mg tissue.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored frozen. RNase inhibitors can be included for prolonged storage.|
The Quick-RNA™ MicroPrep yields high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q & P but not with the Quick-RNA™ MicroPrep. Total RNA was isolated from human epithelial cells (sans DNase treatment).
RNA isolated with the Quick-RNA™ MicroPrep is DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments.
|Step 1||Go here for the updated protocol|
In a study that compared the ability of six commercially available RNA purification kits to extract miRNA from Gyrodactylus salaris, a parasite of freshwater fish such as the Atlantic salmon, the Quick-RNA MicroPrep kit from Zymo Research was shown to result in both the highest yield of miRNA and the best miRNA/total RNA ratio (note: the Quick-RNA MicroPrep kit is an updated version of the ZR RNA MicroPrep kit, which was used in this study). The kit from Zymo Research outperformed the mirVana Kit (Ambion) and the Trizol Plus Kit (Invitrogen).