About Quick-RNA™ FFPE Kit
The Quick-RNA™ FFPE Kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples. The unique chemistries of the product have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin® column technology. The result is high-quality total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.
|Sample Sources||Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2 . It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens.|
|RNA Purity||A260/A280 >1.8, A260/A230 >1.8. DNase I provided for complete removal of DNA.|
|RNA Recovery||The RNA binding capacity of the Zymo-Spin™ IIC Column is ~50 µg.|
|RNA Storage||RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤ -70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage|
|Equipment Needed||Microcentrifuge, vortex, and heat block.|
|RNA Size||RNAs ≥17 nucleotides.|