Quick-RNA™ FFPE Kit

High performance sample prep technology for high quality total RNA (including small/micro RNAs) from FFPE tissue samples and sections.
DNA-free RNA is ready for use in any downstream application. DNase I included.

Product Size Catalog # Price Qty
Quick-RNA™ FFPE Kit 50 Preps R1008
$295.00

About Quick-RNA™ FFPE Kit

The Quick-RNA™ FFPE Kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples. The unique chemistries of the product have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin® column technology. The result is high-quality total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.


Sample Sources Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 cm2 . It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens.
RNA Purity A260/A280 >1.8, A260/A230 >1.8. DNase I provided for complete removal of DNA.
RNA Recovery The RNA binding capacity of the Zymo-Spin™ IIC Column is ~50 µg.
RNA Storage RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage
Equipment Needed Microcentrifuge, vortex, and heat block.
RNA Size RNAs ≥17 nucleotides.

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