About ZR Tissue & Insect RNA MicroPrep™
The ZR Tissue & Insect RNA MicroPrep™ provides for rapid isolation of total RNA from various insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster). The ZR Tissue & Insect RNA MicroPrep™ employs ultra-high density ZR BashingBeads™ for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples.
The Zymo-Spin™ IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IC Column efficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.
RNA can be eluted into volumes as little as 6 µl in less than 10 minutes.
|Format||Bead beating, spin column.|
|Equipment||Microcentrifuge, vortex, cell disrupter/pulverizer (optional).|
|Sample Sources||Small amounts (n ≥ 1 and ≤ 10 mg) of insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster).|
|RNA Purity||High quality total RNA (A260/A280 >1.8, A260/A230 >1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.|
|RNA Recovery||RNA can be eluted into small volumes, ≥6 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~10 µg.|
|Compatibility||Compatible with samples stored in RNAlater™.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.|
Analysis of ZR Tissue & Insect RNA MicroPrep™. Isolation of total RNA from n=2 Drosophila sp. individuals was performed in duplicate (lanes 1 and 2). Samples were processed (2 x 30 sec at 6 m/s) using a FastPrep®-24 Instrument (MP Biomedicals) and resolved alongside (lane M) RNA MilleniumTM Markers (Ambion) in a 1% (w/v) non-denaturing agarose gel.
|Click Here to download the protocol|