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ZR Tissue & Insect RNA MicroPrep™

Quick, 10 minute isolation of total RNA (~10 µg) from insect and arthropod specimens (mosquitoes, bees, lice, ticks, Drosophila melanogaster etc.) using ultra-high density BashingBeads™ and Zymo-Spin™ column technologies.
High-quality RNA eluted in ≥6 µl is ready for reverse transcription, microarray, sequencing, etc.

Product Size Catalog # Price Qty
ZR Tissue & Insect RNA MicroPrep™ 50 Preps. R2030

About ZR Tissue & Insect RNA MicroPrep™

The ZR Tissue & Insect RNA MicroPrep provides for rapid isolation of total RNA from various insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster).  The ZR Tissue & Insect RNA MicroPrep employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples. 

The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IC Column efficiently binds total RNA.  The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.

RNA can be eluted into volumes as little as 6 µl in less than 10 minutes.

Format Bead beating, spin column.
Equipment Microcentrifuge, vortex, cell disrupter/pulverizer (optional).
Sample Sources Small amounts (n ≥ 1 and ≤ 10 mg) of insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster).
RNA Purity High quality total RNA (A260/A280 >1.8, A260/A230 >1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion.
RNA Recovery RNA can be eluted into small volumes, ≥6 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~10 µg.
Compatibility Compatible with samples stored in RNAlater™.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.

Analysis of ZR Tissue & Insect RNA MicroPrep™. Isolation of total RNA from n=2 Drosophila sp. individuals was performed in duplicate (lanes 1 and 2). Samples were processed (2 x 30 sec at 6 m/s) using a FastPrep®-24 Instrument (MP Biomedicals) and resolved alongside (lane M) RNA MilleniumTM Markers (Ambion) in a 1% (w/v) non-denaturing agarose gel.

Click Here to download the protocol
For specific notes and additional information, please see the product protocol PDF.
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