About ZR-96 Viral RNA Kit™
The ZR-96 Viral RNA Kit™provides for rapid, large scale isolation of high-quality viral RNA from a wide range of biological sources. It can be used to successfully isolate viral RNA from cell-free body fluids as well as cellular suspensions at concentrations ≤1x105cells/ml. The kit has been rigorously tested and used to isolate viral RNA from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus and parvovirus (a ssDNA virus).
The ZR-96 Viral RNA Kit™employs a single buffer system that facilitates viral particle lysis and allows for the subsequent RNA adsorption onto the matrix of the Zymo-Spin™I-96 Plate. The RNA is washed then eluted with DNase/RNase-Free Water. The eluted RNA is suitable for use in various subsequent procedures including RT-PCR.
The entire RNA isolation procedure typically takes about 15 minutes.
|Sample Sources||Plasma, serum, culture supernatants, animal cells and tissue.|
|RNA Purity||High-quality RNA suitable for reverse transcription, etc.|
|RNA Recovery||Up to 10 μg RNA can be eluted into as little as 10 μl RNase-free water allowing for a highly concentrated sample.|
|Sample Size||≤200 µl|
|Equipment Needed||Centrifuge with 96-well plate carrier.|
RT-PCR amplification of enterovirus cDNA. Human serum was spiked with different amounts of infectious enterovirus, then viral RNA was extracted using the ZR Viral RNA Kit™. The eluted RNA was used for one-tube RT-PCR amplification of a 230 bp amplicon. M is a 100 bp DNA Marker (Zymo Research)
Add 3 volumes of Viral RNA Buffer to each plasma or serum sample and mix.
Example: Mix 300 µl buffer and 100 µl sample.
Transfer the sample to the Zymo-Spin™ IC Column in a Collection Tube and centrifuge for 1-2 minutes. Discard the flow-through.
Add 500 µl Viral Wash Buffer to the column and centrifuge for 2 minutes. Then carefully transfer the column into the DNase/RNase-Free Tube.
Add 15 µl DNase/RNase-Free Water directly to the column matrix and centrifuge for 30 seconds.
Alternatively, for highly concentrated RNA use ≥6 μl elution.
“The ZR-96 viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”
S.B., Boehringer Ingelheim, Canada