About ZR-96 Quick-RNA™
The ZR-96 Quick-RNA™ kit is an innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Fast-Spin plate technology to yield high quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes.
The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A™ Plate. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.
|Sample Sources||Cells or tissue samples, yeast, plant, bacteria, buccal cells, buffy coat, plasma, serum, and other biological liquids. Compatible with DNA/RNA Shield™ and RNAlater™.|
|Storage||Samples homogenized in RNA Lysis Buffer are stable and can be stored frozen prior to purification.|
|RNA Purity||High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations.|
|RNA Recovery||Up to 10 µg RNA can be eluted into ≥25 µl RNase-free water allowing for a highly concentrated sample.|
|Sample Size||Up to 106 cells or 5 mg tissue.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored frozen. RNase inhibitors can be included for prolonged storage.|
|Equipment Needed||Centrifuge/rotor compatible with 96-well plates.|
The ZR-96 Quick-RNA™ yields high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q & P but not with the ZR-96 Quick-RNA™. Total RNA was isolated from human epithelial cells (sans DNase treatment).
RNA isolated with the ZR-96 Quick-RNA™ is DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments.
|Step 1||Go here for the updated protocol|