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Direct-zol™-96 MagBead RNA

High-throughput, magnetic bead-based purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and all other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.)
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA

Product Size Catalog # Price Qty
Direct-zol™-96 MagBead RNA 2x96 Preps R2100
Direct-zol-96 MagBead RNA w/ TRI Reagent 2x96 Preps R2101
Direct-zol-96 MagBead RNA 4x96 Preps R2102
Direct-zol-96 MagBead RNA w/ TRI Reagent 4x96 Preps R2103
Direct-zol-96 MagBead RNA 8x96 Preps R2104
Direct-zol-96 MagBead RNA w/ TRI Reagent 8x96 Preps R2105

About Direct-zol™-96 MagBead RNA

The Direct-zol-96 MagBead RNA provides a high-throughput, magnetic bead based purification of high-quality RNA directly from samples in TRI Reagent® and similar.  The extraction method inactivates viruses and other infectious agents.  Total RNA including small and non-coding RNAs (17-200 nt) is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product.

The procedure is easy: simply add Direct-zol Binding Buffer and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA.  No phase separation, precipitation, or post-purification steps are necessary.  The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.

*U.S. Patent No. 9,051,563 and other pending patents.

Sample Sources Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.
RNA Purity A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA is performed with DNase I digestion
RNA Recovery The RNA binding capacity is ~10 µg/20 µl MagBinding Beads.
RNA Storage RNA eluted with the DNase/RNase-Free Water (provided) can be stored at ≤-70°C. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage.
Equipment Needed Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.
RNA Size RNAs ≥17 nucleotides.
Sample Inactivation TRI Reagent® (provided only with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents.

Figure 1. Reproducible Sample Processing.
Comparison between manual and automated (Freedom EVO®, Tecan) sample processing with the Direct-zol™-96 MagBead RNA across a 96-well plate. RNA was purified from human epithelial cells (5x 105/well).
Figure 2. High Quality RNA.
RNA quality assessed using a Bioanalyzer. RNA was purified from human epithelial cells using the Direct-zol™-96 MagBead RNA on Freedom EVO® (Tecan).
Figure 3. Efficient Small RNA Recovery.
Small RNA recovery with the Direct-zol™-96 MagBead RNA. Bioanalyzer (Small RNA Chip) gel image shown.

Step 1 Go here for the updated protocol
For specific notes and additional information, please see the product protocol PDF.
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