About Direct-zol™-96 MagBead RNA
The Direct-zol™-96 MagBead RNA provides a high-throughput, magnetic bead based purification of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small and non-coding RNAs (17-200 nt) is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product.
The procedure is easy: simply add Direct-zol™ Binding Buffer and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
*U.S. Patent No. 9,051,563 and other pending patents.
|Sample Sources||Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.|
|RNA Purity||A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA is performed with DNase I digestion|
|RNA Recovery||The RNA binding capacity is ~10 µg/20 µl MagBinding Beads.|
|RNA Storage||RNA eluted with the DNase/RNase-Free Water (provided) can be stored at ≤-70°C. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage.|
|Equipment Needed||Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.|
|RNA Size||RNAs ≥17 nucleotides.|
|Sample Inactivation||TRI Reagent® (provided only with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Step 1||Go here for the updated protocol|