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Direct-zol™-96 RNA

Quick, 96-well purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and all other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.

Product Size Catalog # Price Qty
Direct-zol™-96 RNA 2x96 preps. R2054
Direct-zol™-96 RNA w/ TRI Reagent® 2x96 preps. R2055
Direct-zol™-96 RNA 4x96 preps. R2056
Direct-zol™-96 RNA w/ TRI Reagent® 4x96 preps. R2057

About Direct-zol™-96 RNA

The Direct-zol-96 RNA provides a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®1.  Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product.  The extraction method inactivates viruses and other infectious agents2.

The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I­96 Plate, then spin, wash, and elute the RNA.  No phase separation, precipitation, or post-purification steps are necessary.  The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.

The entire procedure typically takes about 30 minutes (per 2 plates).

*U.S. Patent No. 9,051,563 and other pending patents.

Equipment Centrifuge with microplate carriers.
Sample Sources Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.
Inactivation TRI Reagent® (provided only with R2055 & R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
RNA Purity A260/A280 > 1.8, A260/A230 > 1.8. Complete removal of DNA can be performed with DNase I digestion.
RNA Recovery Typically, RNA is eluted into ≥10 µl DNase/RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the Zymo-Spin™ I-96 Plate is 10 µg/well.
Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
RNA Storage RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage.
RNA Size Limits RNAs ≥17 nucleotides.

Step 1 Go here for the updated protocol
For specific notes and additional information, please see the product protocol PDF.
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“I am very impressed with the RNA yield (quality and quantity) I obtained using this kit. When using other methods and kits for RNA isolation- despite us taking several steps and measures to reduce RNA degradation- I found it very challenging to obtain good quality RNA. With Direct-zol RNA mini prep I was able to obtain superior quality RNA repeatedly and with ease. This kit is suitable for both small scale and high throughput RNA isolations.”
Subashini N. (Wayne State University)
“The protocol very straight forward, time needed to get the RNA is just outstanding and saved us a lot of time to do more things. The yield and the purity of the RNA is something out of this world, about at least 50% more RNA and purity not less than 1.95, we never got [that] before with other vendors such Invitrogen, Qiagen, etc.”
Ehab S. (University of Iowa)
“I liked that it wasn't necessary to separate phases with Chloroform - I think I got less gDNA contamination as a result. The option for performing an on-column DNAse1 digestion is perhaps the most significant benefit compared to doing ChCl3 separations and subsequent precipitations”
Nick A. (AgResearch, New Zealand)
“It is very easy to use and centrifugations are short. The resin is compact and absorbent which makes it easy to cover the whole resin surface with small amount of water in the elution step. Recovery of small RNAs is very good based on CT values obtained from qPCR using RNA template derived from this kit.”
Janne T. (Universidad de Zaragoza)
“The speed of the process is by far the best aspect of this kit. Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? What I also really liked is the ease of the whole process: no messing around with several buffers or different columns that get switched up. Just one column and two buffers, I love it. Oh, and the RNA yield was great too.”
Arjan V. (Indiana University)
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
Adina B. (University of Guelph)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
Mohan K. (University of Illinois, Chicago)
“Easy to use, equal results as a much more expensive kit from another company.”
Patricia O. (University of Porto)
Click here to submit your review of the Direct-zol™-96 RNA.

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