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Reduced Representation Hydroxymethylation Profiling

Reduced Representation Hydroxymethylation Profiling (RRHP)

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Click to view Reduced Representation Hydroxymethylation Profiling Comparison


Strong positive-display method avoids the need for parallel or subtractive sequencing runs


Strand-specific, single nucleotide display of 5-hmC


Small sample input (down to 100 ng)


Simultaneous detection of SNPs


Convenient overlay with methylation data from Zymo's Genome-Wide DNA Methylation Services

RRHP Overview

Click to view our webinar on 5mC and 5-hmC Sequencing

This service is for genome-wide profiling of 5-hydroxymethylcytosine in DNA at single-nucleotide resolution. RRHP also allows strand-specific determination of the location of the 5-hmC modification as well as quantification of 5-hmC levels. Data from RRHP is easily integrated with DNA methylation data from Methyl-MiniSeq™ (previous page), allowing for direct comparison of DNA methylation and hydroxymethylation in the same sample. Omission of chemical conversion processes allows for both low DNA input as well as straightforward SNP detection.

For pricing information and a personalized quote please fill out a service inquiry form or call us at (949) 679-1190 or toll-free at (888) 882-9682.

Services are customizable and can be combined to suit your needs!

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