Reduced Representation Hydroxymethylation Profiling

Reduced Representation Hydroxymethylation Profiling (RRHP)

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Click to view Reduced Representation Hydroxymethylation Profiling Comparison

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Strong positive-display method avoids the need for parallel or subtractive sequencing runs

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Strand-specific, single nucleotide display of 5-hmC

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Small sample input (down to 100 ng)

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Simultaneous detection of SNPs

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Convenient overlay with methylation data from Zymo's Genome-Wide DNA Methylation Services



RRHP Overview

Click to view our webinar on 5mC and 5-hmC Sequencing

This service is for genome-wide profiling of 5-hydroxymethylcytosine in DNA at single-nucleotide resolution. RRHP also allows strand-specific determination of the location of the 5-hmC modification as well as quantification of 5-hmC levels. Data from RRHP is easily integrated with DNA methylation data from Methyl-MiniSeq™ (previous page), allowing for direct comparison of DNA methylation and hydroxymethylation in the same sample. Omission of chemical conversion processes allows for both low DNA input as well as straightforward SNP detection.


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Services are customizable and can be combined to suit your needs!


Click here or contact us at services@zymoresearch.com to inquire today.