LEARN MORE ABOUT EPIQUEST
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EpiQuest Genomic Service
Single Base Resolution DNA Methylation Analysis
Genome-wide coverage: ~1,500,000 CpG Sites, >17,000 Gene Promoters, >21,500 CpG Islands!
How does the EpiQuest™ Genomic Service work?
EpiQuest™ Genome-Wide Service expands upon the conventional RRBS (Reduced-Representation Bisulfite Sequencing) method to greatly increase sequence coverage. Genomic DNA is first digested by restriction endonuclease enzymes to produce fragments rich in CpG sites. These fragments are recovered, subject to bisulfite conversion, and sequenced to give single nucleotide resolution sequencing data. This provides a statistically significant representation of CpG dinucleotides throughout the genome. Through the utilization of a multiple enzyme digestion and an optimized workflow (see below), the EpiQuest™ team at Zymo Research has been able to expand coverage to ≥ 75% of gene promoters and ≥ 80% of CpG islands (for human samples).
The EpiQuest™ Whole-Genome Service utilizes a streamlined library preparation and sophisticated alignment algorithms to deliver Whole Genome Bisulfite-Sequencing that covers > 75% of all the bases in the human genome at single-base resolution.
EpiQuest™ Genomic Service - Platform Details
For pricing information and a personalized quote please fill out a service inquiry form or call us at (949) 679-1190 or toll-free at (888) 882-9682.
Other EpiQuest™ services are now available including locus-specific validation via direct bisulfite sequencing, and global 5mC and 5hmC quantitation by mass spectroscopy determination... please inquire.
