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qMethyl Calculator - For determination of % methylation from duplicate samples

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qMethyl Calculator - For determination of % methylation from duplicate samples


Sample ID	Well	Ct 1st Replicate	Well	Ct 2nd Replicate	Ct Avg		Sample ID	Delta Ct	% Methylation
Test Control 1	A1		A2				Control 1
Reference Control 1	B1		B2				Control 2
Test Control 2	A3		A4				Sample 1
Reference Control 2	B3		B4				Sample 2
Test Sample 1	A5		A6				Sample 3
Reference Sample 1	B5		B6				Sample 4
Test Sample 2	A7		A8				Sample 5
Reference Sample 2	B7		B8				Sample 6
Test Sample 3	A9		A10				Sample 7
Reference Sample 3	B9		B10				Sample 8
Test Sample 4	A11		A12				Sample 9
Reference Sample 4	B11		B12				Sample 10
Test Sample 5	C1		C2				Sample 11
Reference Sample 5	D1		D2				Sample 12
Test Sample 6	C3		C4				Sample 13
Reference Sample 6	D3		D4				Sample 14
Test Sample 7	C5		C6				Sample 15
Reference Sample 7	D5		D6				Sample 16
Test Sample 8	C7		C8				Sample 17
Reference Sample 8	D7		D8				Sample 18
Test Sample 9	C9		C10				Sample 19
Reference Sample 9	D9		D10				Sample 20
Test Sample 10	C11		C12				Sample 21
Reference Sample 10	D11		D12				Sample 22
Test Sample 11	E1		E2
Reference Sample 11	F1		F2
Test Sample 12	E3		E4
Reference Sample 12	F3		F4
Test Sample 13	E5		E6
Reference Sample 13	F5		F6
Test Sample 14	E7		E8
Reference Sample 14	F7		F8
Test Sample 15	E9		E10
Reference Sample 15	F9		F10
Test Sample 16	E11		E12
Reference Sample 16	F11		F12
Test Sample 17	G1		G2
Reference Sample 17	H1		H2
Test Sample 18	G3		G4
Reference Sample 18	H3		H4
Test Sample 19	G5		G6
Reference Sample 19	H5		H6
Test Sample 20	G7		G8
Reference Sample 20	H7		H8
Test Sample 21	G9		G10
Reference Sample 21	H9		H10
Test Sample 22	G11		G12
Reference Sample 22	H11		H12