{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e Competent Cells for Transformation","description":"\u003cp\u003e\u003cspan\u003eBacterial transformation is one of the most fundamental techniques in molecular biology. This method facilitates the transfer of exogenous DNA into a host cell. Once within the cell, the DNA can be used for integration into the genome, replication, protein production, and more. However, not all bacteria can take up exogenous DNA from their environment. For successful DNA uptake to occur, the bacterial cells must be competent.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAt Zymo Research, we have developed \u003cem\u003eMix \u0026amp; Go! E. coli\u003c\/em\u003e Competent Cells that are optimized for simple and high-efficiency transformations, eliminating the need for heat shock and lengthy incubation steps that are associated with standard procedures.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003eMix \u0026amp; Go! E. coli\u003c\/em\u003e Competent Cells enable a simple 20 second transformation process that can help you save over 1 hour on transformations. The competent cells have exceptional transformation efficiencies, reaching up to 10\u003csup\u003e9\u003c\/sup\u003e transformants per µg of plasmid DNA. We have five premade strains available, including the renowned DH5 alpha competent cells. Find the perfect strain of \u003cem\u003eMix \u0026amp; Go!\u003c\/em\u003e Competent Cells for your experiment or produce your own using our \u003ca href=\"\/products\/mix-and-go-e-coli-transformation-kit\" title=\"Mix \u0026amp; Go! E.coli Transformation Kit | ZYMO RESEARCH\"\u003e\u003cem\u003eMix \u0026amp; Go! E. coli\u003c\/em\u003e Transformation Kit and Buffer Set\u003c\/a\u003e. Sample strains and products are available upon request. Unlock the pathway to success in your research and therapeutic development endeavors today.\u003c\/p\u003e","products":[{"product_id":"mix-and-go-competent-cells-jm109","title":"Mix \u0026 Go! Competent Cells - JM109","description":"The \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e strains are premade, chemically competent cells for simple and highly efficient DNA transformation. \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e The premade \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e competent cells are highly efficient (\u0026gt; 10\u003csup\u003e8\u003c\/sup\u003e transformants\/ µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.\n\u003cbr\u003e\u003cbr\u003e\u003ca href=\"\/pages\/mix-and-go-competent-cells\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cu\u003eLearn More\u003c\/u\u003e\u003c\/a\u003e","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix and Go!\u003c\/i\u003e Competent Cells - JM109 \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298445799506,"sku":"T3003","price":155.0,"currency_code":"USD","in_stock":true},{"title":"\u003ci\u003eMix and Go!\u003c\/i\u003e Competent Cells - JM109 \/ 96 x 50 \u0026micro;l \/ No Delay","offer_id":32298445832274,"sku":"T3005","price":594.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/files\/1_Mix-_-Go-Competent-Cells---DH5-Alpha_T3003.gif?v=1725493959"},{"product_id":"mix-and-go-competent-cells-dh5-alpha","title":"Mix \u0026 Go! Competent Cells - DH5 Alpha","description":"The \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e Competent Cells -  DH5 Alpha are premade \u003ci\u003eE. coli\u003c\/i\u003e DH5 alpha competent cells for simple and highly efficient DNA transformation.\nThese DH5 alpha competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e The premade \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e competent cells are highly efficient (\u0026gt; 10\u003csup\u003e8\u003c\/sup\u003e transformants\/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.\n\u003cbr\u003e\u003cbr\u003e\u003ca href=\"\/pages\/mix-and-go-competent-cells\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cu\u003eLearn More\u003c\/u\u003e\u003c\/a\u003e","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e Competent Cells - DH5 Alpha \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298446487634,"sku":"T3007","price":155.0,"currency_code":"USD","in_stock":true},{"title":"\u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e Competent Cells - DH5 Alpha \/ 96 x 50 \u0026micro;l \/ No Delay","offer_id":32298446520402,"sku":"T3009","price":594.0,"currency_code":"USD","in_stock":true},{"title":"\u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e Competent Cells - DH5 Alpha w\/ 96-well PCR plates and Cover Foils \/ 96 x 50 \u0026micro;l \/ No Delay","offer_id":32298446553170,"sku":"T3010","price":594.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/files\/1_Mix-_-Go-Competent-Cells---DH5-Alpha_T3007.gif?v=1725494023"},{"product_id":"mix-and-go-competent-cells-hb101","title":"Mix \u0026 Go! Competent Cells - HB101","description":"The \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e strains are premade, chemically competent cells for simple and highly efficient DNA transformation. \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e The premade \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e competent cells are highly efficient (\u0026gt; 10\u003csup\u003e8\u003c\/sup\u003e transformants\/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.\n\u003cbr\u003e\u003cbr\u003e\u003ca href=\"\/pages\/mix-and-go-competent-cells\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cu\u003eLearn More\u003c\/u\u003e\u003c\/a\u003e","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e Competent Cells - HB101 \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298446717010,"sku":"T3011","price":155.0,"currency_code":"USD","in_stock":true},{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e Competent Cells - HB101 \/ 96 x 50 \u0026micro;l \/ No Delay","offer_id":32298446749778,"sku":"T3013","price":594.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/files\/1_Mix-_-Go-Competent-Cells---DH5-Alpha_T3011.gif?v=1725494091"},{"product_id":"mix-and-go-competent-cells-tg1","title":"Mix \u0026 Go! Competent Cells - TG1","description":"\u003cp\u003eThe \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e strains are premade, chemically competent cells for simple and highly efficient DNA transformation. \u003ci\u003eMix \u0026amp; Go! E. coli\u003c\/i\u003e cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e The premade \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e competent cells are highly efficient (\u0026gt; 10\u003csup\u003e8\u003c\/sup\u003e transformants\/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener noreferrer\" href=\"\/pages\/mix-and-go-competent-cells\" target=\"_blank\"\u003e\u003cu\u003eLearn More\u003c\/u\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e Competent Cells - TG1 \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298446913618,"sku":"T3017","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/files\/1_Mix-_-Go-Competent-Cells---DH5-Alpha_T3017.gif?v=1725494197"},{"product_id":"mix-and-go-competent-cells-zymo-10b","title":"Mix \u0026 Go! Competent Cells - Zymo 10B","description":"The \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e strains are premade, chemically competent cells for simple and highly efficient DNA transformation. \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e \u003ci\u003eE. coli\u003c\/i\u003e cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e The premade \u003ci\u003eMix \u0026amp; Go!\u003c\/i\u003e competent cells are highly efficient (\u0026gt; 10\u003csup\u003e8\u003c\/sup\u003e transformants\/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.\n\u003cbr\u003e\u003cbr\u003e\u003ca href=\"\/pages\/mix-and-go-competent-cells\" target=\"_blank\" rel=\"noopener noreferrer\"\u003e\u003cu\u003eLearn More\u003c\/u\u003e\u003c\/a\u003e","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e Competent Cells - Zymo 10B \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298447077458,"sku":"T3019","price":155.0,"currency_code":"USD","in_stock":true},{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e Competent Cells - Zymo 10B \/ 96 x 50 \u0026micro;l \/ No Delay","offer_id":32298447110226,"sku":"T3020","price":594.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/files\/1_Mix-_-Go-Competent-Cells---DH5-Alpha_T3019.gif?v=1726782423"},{"product_id":"mix-and-go-competent-cells-xjade3-autolysis","title":"Mix \u0026 Go! XJa (DE3) Autolysis Competent Cells","description":"While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of \u003ci\u003eE. coli\u003c\/i\u003e cells. The \u003ci\u003eE. coli\u003c\/i\u003e XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e XJa (DE3) Autolysis Competent Cells, 1 ml 500x L-Arabinose \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298447470674,"sku":"T3031","price":263.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/products\/T3031_XJa_20DE3_20Autolysis_20-_20Glycerol_20Stock_20-_201_20ml_20x_2010.png?v=1588502014"},{"product_id":"mix-and-go-competent-cells-xjbde3-autolysis","title":"Mix \u0026 Go! XJb (DE3) Autolysis Competent Cells","description":"While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of \u003ci\u003eE. coli\u003c\/i\u003e cells. The \u003ci\u003eE. coli\u003c\/i\u003e XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.","brand":"ZYMO RESEARCH","offers":[{"title":"\u003ci\u003eMix \u0026 Go!\u003c\/i\u003e XJb (DE3) Autolysis Competent Cells, 1 ml 500x L-Arabinose \/ 10 x 100 \u0026micro;l \/ No Delay","offer_id":32298447732818,"sku":"T3051","price":263.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/products\/T3051_XJb_20DE3_20Autolysis_20-_20Glycerol_20Stock_20-_201_20ml_20x_2010.png?v=1588502019"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0016\/3731\/8726\/collections\/T3013_Mix-and-Go-Competent-Cells-HB101.jpg?v=1588502747","url":"https:\/\/www.zymoresearch.com\/collections\/mix-go-competent-cells.oembed?page=2","provider":"ZYMO RESEARCH","version":"1.0","type":"link"}