Go From Sample Collection Directly to PCR with DNA/RNA Shield DirectDetect
DirectDetect Eliminates the Need for RNA Extraction in COVID-19 Testing Workflows
Eliminates the need for RNA (or DNA) extraction, allowing for rapid, direct, cost-effective analysis of samples
No inhibition of real-time PCR
Reduces sample viscosity to minimize pipetting errors with automated liquid handlers
Does Not Inhibit PCR-Based Testing
Compared to other reagents used for collecting swab samples, such as saline, DNA/RNA Shield — DirectDetect does not inhibit downstream real-time PCR.
Swabs were collected into either DNA/RNA Shield — DirectDetect or 0.9% saline, and then spiked with heat inactivated SARS-CoV-2 at a concentration of 5,000 copy/mL. 10µl of each sample was used as input into the Quick SARS-CoV-2 Multiplex Kit (R3013) for real-time PCR analysis.
How It Works
Collect sample and mix
Dispense for RT-PCR