How does the Extraction Challenge work?
Zymo Research will send you a 10-prep ZymoBIOMICS DNA Miniprep Kit and one tube of the accurately defined ZymoBIOMICS Microbial Community Standard. Using the ZymoBIOMICS DNA Miniprep and your current extraction method, please extract DNA from the Microbial Community Standard and from a sample of your choice (i.e. environmental sample, feces samples, etc, see below).
|Sample #||Experimental Design|
|1||Microbial Standard (75µl) + ZymoBIOMICS DNA Miniprep|
|2||Microbial Standard (75µl) + ZymoBIOMICS DNA Miniprep|
|3||Your sample of interest (≤100mg) + ZymoBIOMICS DNA Miniprep|
|4||Your sample of interest (≤100mg) + ZymoBIOMICS DNA Miniprep|
|5||Microbial Standard (75µl) + Your DNA extraction method|
|6||Microbial Standard (75µl) + Your DNA extraction method|
|7||Your sample of interest (≤100mg) + Your DNA extraction method|
|8||Your sample of interest (≤100mg) + Your DNA extraction method|
Once the DNA is extracted, please ship to Zymo Research on dry ice or blue ice packs. Please note, that participants will be responsible for all shipping costs associated with sending the DNA samples to Zymo Research. In return, Zymo Research will take care of 16S library prep, sequencing, and bioinformatics analysis. You will then receive a complete sequencing report, allowing you to assess any biases introduced in the DNA extraction step.