Any Organism. One rRNA Depletion Solution.

Zymo-Seq RiboFree Total RNA Library Kit

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Compatible with
any Organism
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Suitable for
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Boost High-Value
NGS Reads
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Zymo-Seq RiboFree Total RNA Library Kit

Comprehensive Gene Detection for Every Transcriptome & Beyond

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The Zymo-Seq RiboFree Kit Detects an Exceptional Number of Genes. Total RNA extracted from various species were used as inputs for library preparation using a single standard RiboFree protocol. The libraries were paired-end sequenced. STAR-aligned reads were quantified at the gene level using featureCounts or StringTie.

Maximize Your Sequencing Efficiency

>90% rRNA Depletion Across Organisms
bar graph indicating rRNA depletion across organisms

The Zymo-Seq RiboFree Kit Produces Dense Coverage of Protein Coding Genes. Classification of the STAR-aligned reads was based on Ensembl annotations and RepeatMasker rRNA tracks from UCSC genome browser when applicable.

Unparalleled Multi-Species Compatibility

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RiboFree Universal Depletion Provides Cross-Species Compatibility. The Zymo-Seq RiboFree Total RNA Library Kit is unmatched in cross-species compatibility thanks to an innovative probe-free depletion strategy. Leveraging the robust depletion kinetics, scientists are exploring diverse applications of RiboFree using samples from vertebrates, plants and more.

Suitable for Degraded Samples*

bar chart indicating rRNA depletion

The Zymo-Seq RiboFree Kit Effectively Depletes rRNA from Formalin-Fixed, Paraffin-Embedded (FFPE) Human Tissue RNA. Total RNA (500 ng) from FFPE tissues was used as input per sample with only one modification to the standard protocol: column cleanup using RNA Clean & Concentrator was adopted after depletion. Median values (n=20) are shown in the figure.

*Please contact for additional recommendations.

The Fastest RNA-Seq Library Workflow

Sample to Sequencer in a Single Day
bar graph that shows Zymo-Seq RiboFree Total RNA Library kit has the fastest workflow

The Zymo-Seq RiboFree Kit has the Fastest Workflow from Total RNA to rRNA-depleted, NGS Libraries. The Zymo-Seq RiboFree Total RNA Library Kit offers a ≥ 30% reduction in workflow completion time compared to other available RNA library prep kits. Workflow time considers the hours required to complete both rRNA depletion and library preparation.