image of a cow, chicken, and wheat
Compatible with
Any Organism
image of microscope slides with a purple blob ready for examination
Suitable for
image of a magnifying glass analyzing part of an RNA strand
Boost High-Value
NGS Reads
Zymo-Seq RiboFree Total RNA Library Kit product image

Zymo-Seq RiboFree Total RNA Library Kit

Comprehensive Gene Detection for Every Transcriptome & Beyond

chart indicating number of genes detected in various organism

The Zymo-Seq RiboFree Kit Detects an Exceptional Number of Genes. Total RNA extracted from various species were used as inputs for library preparation using a single standard RiboFree protocol. The libraries were paired-end sequenced. STAR-aligned reads were quantified at the gene level using featureCounts.

Maximize Your Sequencing Efficiency

>90% rRNA Depletion Across Organisms
bar graph indicating rRNA depletion across organisms

The Zymo-Seq RiboFree Kit Produces Dense Coverage of Protein Coding and Other Transcripts. Classification of the STAR-aligned reads was based on Ensembl annotations and RepeatMasker rRNA tracks from UCSC genome browser when applicable.

Unparalleled Multi-Species Compatibility

chart indicating multi-species compatibility using the Zymo-Seq RiboFree Total RNA Library Kit

RiboFree Universal Depletion Provides Cross-Species Compatibility. The Zymo-Seq RiboFree Total RNA Library Kit is unmatched in cross-species compatibility thanks to an innovative probe-free depletion strategy. Leveraging the robust depletion kinetics, scientists are exploring diverse applications of RiboFree using samples from vertebrates, plants and more.

Suitable for Degraded Samples*

bar chart indicating efficient rRNA depletion in human FFPE samples

The Zymo-Seq RiboFree Kit Effectively Depletes rRNA from Formalin-Fixed, Paraffin-Embedded (FFPE) Human Tissue RNA. Libraries were prepared with 250 ng, 100 ng, and 50 ng of total RNA extracted from FFPE tissues (RIN = 1.6, peak < 200 nt), respectively, with minimal modifications to the standard protocol as described in Appendix E of the instruction manual.

*Please contact for additional recommendations.

The Fastest RNA-Seq Library Workflow

Sample to Sequencer in a Single Day
bar graph that shows Zymo-Seq RiboFree Total RNA Library kit has the fastest workflow

The Zymo-Seq RiboFree Kit has the Fastest Workflow from Total RNA to rRNA-depleted, NGS Libraries. The Zymo-Seq RiboFree Total RNA Library Kit offers a 20% - 40% reduction in workflow completion time compared to other available RNA library prep kits. Workflow time considers the hours required to complete both rRNA depletion and library preparation.

Need help? Contact Us