GpC Methylase (M. CviPl) (Discontinued)
E2014 / E2015
- For complete, in vitro methylation of DNA for methylation analysis.
- Methylation of chromatin DNA for DNA accessibility studies.
- Inhibition of endonucleases with overlapping GpC sequence recognition and [3H]-labeling of DNA.
Our GpC Methylase completely methylates all cytosines within a 5′…GpC…3′ context in double-stranded DNA. The enzyme is specific for both non-methylated and hemimethylated DNA. The recombinant GpC Methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Chlorella virus. The reaction conditions are optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.
|Assay Condition||GpC Methylase in 1X GpC Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 37°C.|
|Heat Inactivation||65°C for 20 minutes|
|Storage||Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C.|
|Unit Definition||One unit (U) is defined as the amount of enzyme required to protect 1 µg of λ DNA against cleavage by HaeIII restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C.|