Zymo Research's RNA-Seq service makes Next-Gen transcriptome analysis available to every researcher, without the need for
expensive equipment or bioinformatics expertise. Now you can achieve transcriptome-wide coverage of total RNA, or small RNA
with the latest Next-Gen sequencing technology.
- Gene expression studies
- miRNA analysis
- Non-coding RNA investigations
- Discovering splice variants, SNPs, and RNA editing sites
- And much more!
Let our scientists do the work, starting with RNA purification and sample prep all the way through the bioinformatic analyses with
the delivery of a report with publication-ready figures directly to you. Or, we can perform only the steps you want. Each project is
fully customizable to ensure your needs are met!
Many types of analyses are available including total RNA-Seq, small RNA-Seq (miRNA), polyadenylated RNA-Seq, and non-polyadenylated RNA-Seq.
The Role of Acyl-CoA Synthetase 4 in Aggressive Breast Cancer
Castillo, et al. used the mRNA-Seq service from Zymo Research to investigate gene expression profilse in breast cancer cells overexpressing acyl-CoA synthetase 4 (ACSL4), since the expression of ACSL4 has been linked to aggressive breast cancer.
Ingenuity Pathways Analysis performed on data obtained from mRNA-Seq showed that ACSL4 overexpression affected expression levels of genes involved in tumorigenesis-related biofunctions, with cell movement, migration,
and proliferation being the top three. Furthermore, the results of the reverse phase protein array showed that cells overexpressing ACSL4 have a distinct protein signature.
Based on DAVID analysis, the researchers concluded that ACSL4 can stimulate different signaling pathways, which mediate aggressiveness of breast cancer cells, for example, pathways activated by growth factors, cytokines, and nutrients.
They suggested that ACSL4 could potentially be used to predict an aggressive breast cancer phenotype in patients and could be a target for cancer therapy.
Castillo, A. F., et al.(2015) Gene Expression Profile and Signaling Pathways in MCF-7 Breast Cancer Cells Mediated by Acyl-Coa Synthetase 4 Overexpression. Transcriptomics 3.120 (2015): 2.