About Zymoclean™ Large Fragment DNA Recovery Kit
The Zymoclean™ Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 10 μg total DNA per column can be eluted into ≥ 10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥ 23 kb, the recovery is 50-70%. |
| Equipment | Microcentrifuge |
| DNA Size Limits | 50 bp to ~200 kb |
| Sample Sources | DNA excised from TAE and TBE-buffered agarose gels. |
| DNA Purity | High-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
Recovery of large DNA fragments. The Zymoclean™ Large Fragment DNA Recovery Kit was used to recover λ DNA digested with HindIII and separated by agarose gel electrophoresis. Lane C: λ-HindIII digest; lanes 1 & 3: recovered 23 kb λ-HindIII fragments; lanes 2 & 4: recovered 9 kb λ-HindIII fragments. Lane λ: intact λ phage DNA; lanes 5, 6: intact λ ~48 kb bands.
Your shopping cart is empty.
