About YeaStar™ Genomic DNA Kit
The YeaStar™ Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Fast-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 1.5 hr |
| Equipment | Microcentrifuge |
| DNA Size Limits | > 1 kb |
| DNA Purity | Ultra-pure DNA is recovered in water. Eluted DNA is ideal for use in DNA ligation, sequencing, transcriptions, labeling, restriction endonuclease digestion, etc. |
| Recovery Volume | ≥ 60 µl |
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Agarose gel electrophoresis of DNA prepared using the YeaStar™ Genomic DNA Kit. Lanes: M: ?-DNA Hind III marker; 1: S. cerevisiae; 2: P. pastoris; 3: C. albicans; 4: S. pombe.
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