About ZR Urine DNA Isolation Kit™
The ZR Urine DNA Isolation Kit™ is an innovative product designed for the easy, reliable, and rapid isolation of total DNA from cells and biological sediment in urine samples. The product enables isolation of cells from urine using a syringe fitted with a uniquely-designed syringe filter. Following separation, cells are lysed and the collected lysate can be processed immediately or at a later time following transportation and/or storage. The DNA isolation procedure is simple and can be performed in less than 10 minutes with the technologies featured in this kit. Total DNA isolated with the ZR Urine DNA Isolation Kit™ is ideal for PCR, array, methylation detection, etc.
Specifications
| Format | Spin Column |
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| Equipment | Syringe (30 ml, 1 ml), Microcentrifuge |
| DNA Size Limits | 100 bp to ≥ 40 kb |
| Sample Sources | Urine |
| DNA Purity | High quality DNA for PCR, bisulfite treatment/methylation detection, array, etc., is eluted with DNA Elution Buffer or water. (A260/A280>1.8) |
| Sample Size | 30 ml per standard preparation. The sampled volume can be scaled up or down if required. |
| DNA Yield | The DNA binding capacity of the column is 5 µg. Female urine typically yield 14-200 ng DNA/ml. Male urine yield 4-60 ng DNA/ml. |
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DNA purified from human urine using the ZR Urine DNA Isolation Kit™ is ideal for use in PCR. The gel image above shows the results of PCR amplification of 15 ng, 1.5 ng, and 0.15 ng total DNA (1x, 10x 100x dilutions, respectively) isolated from human urine using primers specific for the human ß-actin gene. (M) is a 50 bp DNA ladder (Zymo Research) and amplicons are indicated (?). The (+) and ("“) are positive and negative controls, respectively.
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