About ZR Viral DNA Kit™
The ZR Viral DNA Kit™ provides for the rapid isolation of high-quality viral DNA from a wide range of biological sources. A uniquely designed buffer is included for the efficient denaturation of viral particles in whole blood (fresh and stored), plasma, serum, tissue, ascites, cultured cells, and from liquid samples. DNA can be eluted with elution buffer or water and is suitable for subsequent PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 5 μg total DNA is eluted into 6-10 μl DNA Elution Buffer. For DNA 75 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 50 kb the recovery is 50-70%. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex |
| DNA Size Limits | 100 bp to 50 kb. |
| Sample Sources | Whole blood, serum, plasma, tissue, or cells derived from humans, mice, rabbits, etc. |
| DNA Purity | High quality DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 >1.8 |
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Viral DNA purification. Human HBV DNA was isolated from 10 to 0.001 µl of human serum using phenol/chloroform or ZR Viral DNA Kit™. The presence of HBV DNA is evidenced by a ~200 bp PCR amplicon. Lane M is a 100 bp DNA Ladder and "Neg." is the negative control for PCR.
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