About EZ Nucleosomal DNA Prep Kit
The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of mammalian and yeast nucleosome-associated DNA. The kit includes procedures and reagents for: cell nuclei isolation, intact nuclei micrococcal nuclease digestion, and nucleosomal DNA purification.
Non-nucleosomal DNA is specifically degraded using micrococcal nuclease and an optimized reaction buffer; while purification of "protected" nucleosome-associated DNA is performed using Zymo Research's proven Fast-Spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 30 minutes!
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Sample Sources | Nucleosome associated DNA isolation and purification from mammalian and yeast cells. |
| DNA Purity | High quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR amplification, arrays, Southern blot analysis, DNA quantification, sequencing, and other molecular applications. |
| Product Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Mammalian Nucleosomal DNA Preparation: Mammalian nuclei prepared as indicated by the Mammalian Nuclei Prep Protocol (see opposing column) was treated with 0.05 and 0.5 U (unit) micrococcal nuclease for the indicated times (min) at 25°C. DNA was subsequently resolved in a 2% agarose gel. 100 bp DNA ladder (Zymo Research). Asterisks (1N, 2N, 3N) represent mono-, di-, and tri-nucleosomal DNAs, respectively.
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