About ZR-96 Plant/Seed DNA Kit™
The ZR-96 Plant/Seed DNA Kit™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Fast-Spin plate technology. The eluted DNA is filtered to remove polyphenolics making it ideal for downstream molecular-based applications including PCR, arrays, etc.
Specifications
| Format | 96-well Plate |
|---|---|
| DNA Recovery | Up to 5 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
| Processing Time | 50 min |
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer. |
| DNA Size Limits | Capable of recovering genomic DNA sized fragments from 100 bp to ≥ 40 kb. Typical fragment sizes range from 25 kb "“ 35 kb. If present, parasitic and viral DNA will also be recovered. |
| Sample Sources | Up to 80 mg of plant sample sources which include leaves, stems, buds, flowers, fruit, seeds, etc. |
| DNA Purity | High quality, inhibitor-free DNA is eluted with DNA Elution Buffer suitable for the amplification of bacterial, protist, and/or mammalian templates. Absorbance 260 nm/280 nm >1.8. |
| DNA Yield | Typically 20-80 ng DNA/mg plant material is recovered. |
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Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA MiniPrep™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research). Arabidopsis thaliana (1), juniper (2), corn kernel (3, 4), sunflower seed (5, 6).
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