About ZR Plant/Seed DNA MiniPrep™
The ZR Plant/Seed DNA MiniPrep™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Fast-Spin column technology. The eluted DNA is filtered to remove polyphenolics making it ideal for downstream molecular-based applications including PCR, arrays, etc.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Up to 25 μg total DNA is eluted into ≥ 25 μl DNA Elution Buffer per sample. |
| Processing Time | 15 Min |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer (optional) |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | Up to 150 mg that include leaves, stems, buds, flowers, fruit, seeds, etc. |
| DNA Purity | High quality, inhibitor-free DNA is eluted with DNA Elution Buffer that is suitable for PCR amplification. A260/A280 > 1.8 |
| DNA Yield | Typically 20-80 ng DNA/mg plant material is recovered. |
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Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA MiniPrep™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research). Arabidopsis thaliana (1), juniper (2), corn kernel (3, 4), sunflower seed (5, 6).
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