About ZR Soil Microbe DNA MicroPrep™
The ZR Soil Microbe DNA MicroPrep™ is designed for the simple and rapid isolation of humic-free, PCR-quality DNA from microbes in soil. This product can be used to isolate DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a variety of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil microbes are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. The procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA is eluted into ≥ 10 µl DNA Elution Buffer per sample. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer (optional) |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | DNA is isolated from bacteria, fungi, protozoa, and algae in up to 0.25 g of soil or from fungal/bacterial cells directly. |
| DNA Purity | High-quality, humic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 >1.8 |
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Metagenomic DNA isolated from 5 soil samples. M: 1 kb marker (NEB); 1-5: soil samples (sand, sandy clay loam, hydrophobic sandy loam, course sandy loam, fine gravel).
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The ZR Soil Microbe DNA Kit provided the best results of the commercially available kits!--Sven Aerts, Elke Jacops, An Dewel (SCK.CEN)
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