About Pinpoint Slide DNA Isolation System™
The Pinpoint™ Slide DNA Isolation System is an innovative product for the isolation of total DNA from targeted areas of fresh, frozen, and FFPE tissue sections. There is no need for expensive specialized equipment or computer software. Instead, the system combines innovative Pinpoint™ tissue sampling technology, Proteinase K digestion, and a one-step DNA extraction method for the isolation of DNA that is ideal for PCR, sequencing, etc.
| Format | Spin Column |
|---|---|
| Equipment | Microcentrifuge, vortex, centrifuge w/ microplate carriers |
| DNA Size Limits | 75 bp - 25 kb |
| Sample Sources | Fresh or paraffin-embedded tissue sections on glass slides. |
| DNA Purity | Genomic DNA that is especially well suited for PCR, sequencing, etc. |
| Tissue Recovery Area | 0.5 cm2 |
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| Step 1 | Preparation of Proteinase K working stock: Simply add 260 µl Proteinase K Storage Buffer to the Proteinase K tube. Dissolve completely and store at -20°C. |
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| Step 2 | Add 14 ml of 95-100% ethanol to the PP Wash Buffer Concentrate to make final PP Wash Buffer. |
For specific notes and additional information, please see the product protocol PDF.
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