About Xpedition™ Tissue & Insect DNA MiniPrep
Degradation and contamination of biological samples have been obstacles to scientific study, and may be particularly problematic in highly sensitive molecular-analysis techniques (e.g., PCR of low copy DNA). Use of cryogenic freezing methods for environmental/forensic sample preservation may often be too impractical to be employed. The Xpedition™ Tissue & Insect DNA MiniPrep, when used with a portable bead beating device [i.e., Xpedition™ Sample Processor (XSP), pg. 6], allows the researcher/investigator to "Take the Lab to the Field" and to the site of sample collection.
The Xpedition™ Tissue & Insect DNA MiniPrep can be used for the isolation of inhibitor-free DNA from tough-to-lyse tissues and small organisms (insects). Samples from remote locations can be processed at the site of collection using the provided ZR BashingBead™ Lysis Tubes and a XSP (available separately, Cat. No. S6020, pg. 6). Sample processing occurs in a unique lysis/stabilization solution that effectively preserves DNA in tissue lysates for subsequent storage and transport. Later, when convenient with the researcher, the kit's Fast-Spin column technologies are used for the isolation of inhibitor-free DNA from the lysates. Eluted DNA is ideal for PCR for genotypic analysis including metagenomics, barcoding, phylogenetics, forensics, etc. An overview of the procedure is shown below.
Specifications
| Format | Bead Beating, Spin Column |
|---|---|
| DNA Recovery | Typically, up to 25 µg total DNA is eluted into 100 µl (25 µl minimum) DNA Elution Buffer per sample. |
| Equipment | Microcentrifuge, vortex, cell disrupter/pulverizer (optional) |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | Samples (n ≥ 1 and ≤ 50 mg) of fresh, frozen, or stored insects including: mosquitoes, bees, lice, ticks, D. melanogaster, etc. Also, compatible with fresh or frozen mammalian tissues as well as cultured cells, and whole blood. |
| DNA Yield | Expected yields can range from 1 - 5 µg DNA per mg specimen sampled. For mammalian tissues, yields are 1 - 3 µg DNA per mg of skeletal, heart and brain tissues and 3 - 5 µg DNA per mg of liver, kidney, and lung tissues. Whole blood will yield from 3 - 7 µg DNA per 100 µl. |
| Purity | High quality DNA is eluted with DNA Elution Buffer that is well suited for PCR amplification, endonuclease digestion, etc. A260/A280 >1.8 |
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