About ZR Genomic DNA™ -Tissue MicroPrep
The ZR Genomic DNA™-Tissue MicroPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, parasitic, microbial, viral) from a variety of solid tissues. The product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and are also compatible with inputs including: buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K then add the Genomic Lysis Buffer, vortex, and transfer the mixture to the supplied spin column. PCR inhibitors are effectively removed during the purification process and purified DNA is suitable for downstream applications including: PCR, Southern blotting, DNA sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, etc.
Specifications
| Format | Spin Column |
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| DNA Size Limits | Capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25 kb - 35 kb. |
| Sample Sources | Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources are effectively processed using this kit. |
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High yield/quality DNA is successfully isolated from porcine muscle using the ZR Genomic DNA™-Tissue MiniPrep. Equivalent amounts (25 mg) of muscle tissue were processed using the ZR Genomic DNA™ Tissue MiniPrep after incubation with Proteinase K at 55ºC for the indicated times (in minutes) or overnight (O/N). Equal volumes of eluted DNA were analyzed in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. M: 1 kb ladder (Zymo Research).
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