About ZR Tissue & Insect DNA MicroPrep™
The ZR Tissue & Insect DNA MicroPrep™ is designed for the simple and rapid isolation of DNA (e.g., genomic, viral, mitochondrial) from fresh, frozen, or stored insect specimens including mosquitoes, bees, lice, ticks, and D. melanogaster. The procedure is easy and can be completed in minutes: samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. The DNA is then isolated and purified using our Fast-Spin column technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood, and cultured cells.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 5 µg total DNA is eluted into ≥ 10 µl DNA Elution Buffer per sample. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
| DNA Size Limits | > 1 kb |
| Sample Sources | Samples (n ≥ 1 and ≤ 10 mg) of fresh, frozen, or stored insects including: mosquitoes, bees, lice, ticks, D. melanogaster, etc. Also, compatible with fresh or frozen mammalian tissues (e.g., soft tissues like liver and brain) as well as cultured cells, and whole blood. |
| DNA Purity | High quality DNA is eluted with DNA Elution Buffer that is well suited for PCR amplification, endonuclease digestion, etc. A260/A280 >1.8 |
| DNA Yield | Expected yields can range from 1-5 µg DNA per mg specimen sampled. For mammalian tissues, yields are 1-3 µg DNA per mg of skeletal, heart and brain tissues and 3-5 µg DNA per mg of liver, kidney, and lung tissues. Whole blood will yield from 3-7 µg DNA per 100 µl. |
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DNA yields from various insect and mouse samples using the ZR Insect & Tissue DNA MiniPrep™. Various amounts of sample were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The 1 kb DNA size marker is from Zymo Research
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