About Femto™ Fungal DNA Quantification Kit
The Femto™ Fungal DNA Quantification Kit can detect and quantify fungal DNA with high specificity and sensitivity. Fungal DNA can be reliably quantified in a background of non-fungal DNA such as bacterial, animal, plant DNA, etc. This is essential for downstream applications that require accurate DNA input amounts such as quantifying fungal DNA template in order to set up for Next-generation sequencing library preparation and metagenomic analysis. With the Femto™ Fungal DNA Quantification Kit, dependably quantify as little as 20 fg of fungal DNA from 1 µl of purified biological liquids, fungal cultures, or environmental DNA samples.
|Sample Sources||Detect and quantify high quality fungal DNA from any purified mixed DNA sample.|
|Compatibility||Product is designed to be compatible with any real-time and quantitative PCR instrument.|
|Bacterial DNA Detection and Quantification||Detection range of 20 fg-20 ng from as little as 1 µl of sample. The kit can be used to detect down to 2 copies of Saccharomyces cerevisiae genomic DNA.|
Reliable standards for the quantification of fungal DNA: Fungal DNA Standards (measured in duplicates) comprise 10-fold dilution series ranging from 2 ng to 20 fg.
Amplification of fungal DNA from a variety of samples: Amplification plots of the Femto™ Fungal DNA Quantification Kit of inputs of purified DNA extracted from different sources are shown: Chinchilla feces (purple), sludge (light blue), and soil (orange). Fungal DNA Standards (green) and No Template Control (black) are also shown.
✓It is recommended that all reagents and qPCR reactions be prepared using clean techniques to prevent contamination.
✓Femto™ Human qPCR Premix should be completely thawed at room temperature, mixed by flicking the tube, centrifuged briefly, and then placed on ice. DO NOT VORTEX Femto™Human qPCR Premix.
✓Femto™ Human qPCR Premix should be protected from direct light exposure. Minimize freeze-thaw cycles.
✓ Human DNA Standards (#1-7) should be completely thawed at room temperature, mixed by vortexing, centrifuged briefly, and then placed on ice.
✓All reagents should be kept on ice immediately after thawing.
Protocol for Human DNA Quantification:
Aliquoting Femto™ Human qPCR Premix and qPCR Set Up (for qPCR tube strips or qPCR plates)
-Initial Denaturation 95 ˚C 10 minutes
-Denaturation 95 ˚C 30 seconds
-Annealing 59 ˚C 30 seconds 40 cycles
-Extension 72 ˚C 1 minute
-Final Extension 72 ˚C 7 minutes
Use the Human DNA Standards table below to generate a standard curve to quantify Unknown Test Samples. For example, the Standard 1 reaction wells contain 20 ng of human DNA, Standard 2 reaction wells contain 2 ng of human DNA, etc.