Credit Orders: Currently, online ordering is not available via credit card (PO can still be processed).
Please call 888-882-9682 for assistance. We apologize for the inconvenience; use code NEWWEB10 for a 10% discount.

ChIP DNA Clean & Concentrator™

Two (2) minute DNA clean-up from any step in a standard ChIP protocol.
DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.

Product Size Catalog # Price Qty
ChIP DNA Clean & Concentrator™ - Uncapped Columns 50 Preps. D5201
$96.00
ChIP DNA Clean & Concentrator™ - Capped column 50 Preps. D5205
$101.00

About ChIP DNA Clean & Concentrator™

The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP. This kit may be applied to any routine ChIP procedure to determine DNA concentration of samples that have undergone reverse cross-linking following DNA shearing. It can also be used for the removal of TES, 0.1M NaHCO3 and 1% SDS from DNA eluted from chromatin-antibody-bead complexes and can be used to purify DNA from buffers containing up to 1% SDS or 5% NP-40, Tween-20, Triton X-100 or Sarkosyl.


DNA Recovery For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
DNA Size Limits 50 bp to ~ 23 kb
Sample Sources Wherever DNA isolation and purification is required during standard ChIP protocols. This includes samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following either 1) mechanical or nuclease-mediated DNA shearing or 2) elution from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS. This kit can also be used for DNA purification from PCR, enzymatic digestion, kinase, phosphatase and other enzymatic reactions.
DNA Purity High quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR amplification, arrays, Southern blot analysis, DNA quantification, sequencing, and other molecular applications.
Product Detergent Tolerance ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 1% SDS

 

Agarose gel electrophoresis of DNA isolated from cell lysates. High quality DNA can be efficiently recovered from Saccharomyces cerevisiae cell lysates using the ChIP DNA Clean & Concentrator™. Duplicate purifications were performed with 5, 15, and 30 µl cell lysate and an equal volume of eluted DNA was loaded into each lane. The size marker M1 and M2 are 100 bp and 1 kb ladders, respectively (Zymo Research).

Step 1

In a 1.5 ml microcentrifuge tube, add 5 volumes of ChIP DNA Binding Buffer to each volume of sample (5:1).  Mix briefly.

Example 1: Add 250 µl ChIP DNA Binding Buffer to 50 µl cell lysate following DNA shearing, reverse cross-linking and Proteinase K digestion in TES (50 mM Tris-Cl, pH 8.0, 10 mM EDTA, 1% SDS) or 0.1M NaHCO3 containing 1% SDS  .

Example 2: Add 600 µl ChIP DNA Binding Buffer to 120 µl eluent in TES or 0.1M NaHCO3 containing 1% SDS buffers from chromatin-antibody-Protein A agarose-bead complexes followed by reverse cross-linking and Proteinase K digestion. 

Step 2

Transfer mixture to a provided Zymo-Spin™ Column in a Collection Tube.

Step 3

Centrifuge at ≥ 10,000 x g for 30 seconds.  Discard the flow-through.

Step 4

Add 200 µl Wash Buffer to the column.  Centrifuge at ≥ 10,000 x g for 30 seconds.  Repeat wash step.

Step 5

Add 6-100 µl Elution Buffer directly to the column matrix.  Transfer the column to a new 1.5 ml microcentrifuge tube and centrifuge at ≥ 10,000 x g for 30 seconds to elute the DNA.

Ultra-pure DNA is now ready for use for PCR, arrays, DNA quantification, sequencing and other molecular applications.

For specific notes and additional information, please see the product protocol PDF.
Submit your Citation
GETTING PUBLISHED? Take some time to celebrate and enjoy the little things in life!

Getting published is not only hard work, it takes enormous amounts of time and dedication. Zymo Research would like to celebrate your achievement! Let us know when you have cited any of our products in your recent publication and get a $20 Amazon gift code.

Featured Citations

Researchers sought to further understand regulatory mechanisms of the glucocorticoid receptor (GR) and KL15, a zinc-finger transcription factor is directly induced by GR. Chromatin immunoprecipitation was performed using GR and FLAG antibodies followed by DNA purification using the ChIP DNA Clean &Concentrator™. qPCR analysis demonstrated that GR binds to two intronic GR binding regions in the KLF15 locus. Further experiments also demonstrated a feed-forward regulatory mechanism for GR and KLF15. Taken together, the data indicates that GR and KLF15 form coherent and incoherent feed-forward circuits, providing a novel mechanism for temporal control and signal integration by GR.
Researchers set out to determine the role of lamina-associated polypeptide 2α (LAP2α) in the progression of the cellular phenotype of Hutchinson-Gilford progeria syndrome (HGPS), an extremely rare aging disease. LAP2α and laminA/C chromatin immunoprecipitation (ChIP) in progerin-expressing and lamin A-expressing cells was performed. ChIP DNA was purified using the ChIP DNA Clean & Concentrator™ and the association of LAP2α and lamin A/C with extra cellular matrix (ECM) genes down-regulated in progerin-expressing cells was tested by qPCR. LAP2α and lamin A/C bound to both ECM genes in all cells, but interestingly, gene- associated lamin A/C levels were higher in progerin-expressing versus lamin A-expressing and control cells. The results suggest that gain of lamin A association in genomic regions containing ECM genes in progeria cells may contribute to gene repression.
Researchers used the ChIP DNA Clean & Concentrator™ to isolate ChIP DNA for chromatin immunoprecipitation studies to analyze the epigenome-wide effects of a vitamin D metabolite, 1,25(OH)2D3, on chromatin accessibility of human monocytes. The study demonstrated that chromatin sites were not only highly significantly enriched for CTCF binding motifs, but that some of the sites also bound the transcription factor. In summary, the researchers demonstrated that a large number of vitamin D-dependent epigenome-wide events precede and accompany the transcriptional activation of 1,25(OH)2D3 target genes.
Researchers purified the insoluble DNA fraction from the FAIRE assays using a ChIP DNA Clean & Concentrator™ kit (Zymo Research) after reverse cross-linking to investigate the impact of CHD3 on the “chromatin structure” of the HSV genome during early infection. They found that the level of viral genomes in the soluble fraction increases in parallel with a decrease in the level of the insoluble fraction as CHD3 is depleted.
Researchers used the ChIP DNA Clean and Concentrator™ from Zymo Research after RNase and Proteinase K treatments to isolate the DNA immunoprecipitated in their ChIP assay. DNA samples were used to study the binding of AhR to the rat DRE II region located in proximal Jup promoter.
Immunoprecipitated chromatin was purified using the ChIP DNA Clean & Concentrator™ from Zymo Research. Purified DNA was then used as templates for PCR to study the enrichment of histone modifications (e.g. H3K27me3, H3K4me3, H3K9ac, and H3K27ac) and other factors (e.g. EZH2) in the Zfp423 promoter region of fetal tissue from offspring of female mice that were fed either control or obesogenic diets.
Researchers purified immunoprecipitated chromatin from their ChIP assays using the ChIP DNA Clean & Concentrator™ kit from Zymo Research. The purified DNA was used for real-time quantitative PCR analysis to study whether tethered origin recognition complex (ORC) proteins could recruit additional pre-RC proteins, endogenous Mcm7, DUE-B and Cdc45 to the c-myc-GAL4-binding site.
Chromatin immunoprecipitation assays were performed with A549 and Beas-2B cells using GR or FLAG antibodies. ChIPed DNA was purified with the ChIP DNA Clean and Concentrator™ kit from Zymo Research and the purified DNA was used to study the occupancy of KLF15 and GR at the GR-KLF15 response elements by qPCR analysis.
The ChIP DNA Clean & Concentrator™ was used to purify the DNA fragments in DNA–protein complexes precipitated by antibodies against KLF6 or Sp1. Purified DNA was used to analyze the capacity of KLF6 and Sp1 for binding to exon 1 of the tmsg-1 gene.
ChIP assays were carried out from Xenopus embryonic tissues and DNA fragments were purified using ChIP DNA Clean & Concentrator. Purified DNA was used to study the occupancy of TCF proteins on Vent2 promoter.
ChIP DNA was recovered and purified using Zymo's ChIP DNA Clean and Concentrator™ Kit. Purified ChIP DNA was subjected to TaqMan qPCR with specific primers and probes to study the effect of 5-aza-CdR treatment on the association of AP-2alpha at hZip1 and hZip3 promoter regions.
Proteinase K/RNase-treated DNA fragments were purified using ChIP DNA Clean & Concentrator™ spin column and purified DNA was used to analyze the association of VDR and changes of histone H3 and H4 acetylation within the major regulatory modules of the CYP3A4 gene.
Researchers performed a series of ARID1A chromatin immunoprecipitation (ChIP) experiments in primary ovarian surface epithelial cells. ChIPed DNA was purified with the ChIP DNA Clean & Concentrator™ Kit and purified DNA was used to determine the occupancy of ARID1A at the IL-6 promoter. These results, combined with PIK3CA expression data indicate that coexistence of ARID1A-PIK3CA mutations lead to IL-6 upregulation in their ovarian clear-cell carcinoma model.
The authors isolated and concentrated ChIP DNA using the ChIP DNA Clean and Concentrator™ kit after reverse cross-linking step and concentrated ChIP DNA was used to examine the effect of selenium towards histone acetylation. They found that selenium supplementation in the form of selenite decreased the enrichment of H4K12ac and H4K16ac at the COX-2 and TNFα promoters in primary and immortalized macrophages.

Click here to submit your review of the ChIP DNA Clean & Concentrator™.

Call Us

1.888.882.9682

We're here Mon - Fri 8am - 5pm PST

Send an Email

info@zymoresearch.com

We'll reply within 24 hours