Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues
Zymo-Spin™ technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.

Product Size Catalog # Price Qty
Quick-DNA™ Miniprep Plus Kit 10 Preps D4068T
$45.00
Quick-DNA™ Miniprep Plus Kit 50 Preps D4068
$132.00
Quick-DNA™ Miniprep Plus Kit 200 Preps D4069
$489.00

About Quick-DNA™ Miniprep Plus Kit

Note: The Quick-DNA™ Universal Kit (D4068/D4069) has been renamed to Quick-DNA™ Miniprep Plus Kit; all components and protocols are the same and have not been modified.

The Quick-DNA™ Miniprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin Column technologies allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 35 µl.  Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free, bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis. 


Equipment Water bath or heat block (55oC), microcentrifuge, and vortex.
DNA Purity High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230 ≥ 2.0).
Elution Volume DNA can be eluted into as little as 35 µl DNA Elution Buffer or water.
DNA Yield The DNA binding capacity of the column is 25 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
DNA Size Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
DNA Applications DNA isolated using the Quick-DNA™ Miniprep Plus Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications.
DNA Types The Quick-DNA™ Miniprep Plus Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. Not recommended for small cell-free DNA isolation from urine, serum, and plasma.
Workflow Overview Utilize a Proteinase K Digestion and Zymo-Spin™ Column for effective recovery of DNA.

High Quality DNA Obtained from a Wide Range of Biological Samples Using the Quick-DNA™ Miniprep Plus Kit. DNA purified using the Quick-DNA™ Miniprep Plus Kit is ultrapure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).

   

DNA Yields Increase Linearly with Increasing Volumes of Human Whole Blood Using the Quick-DNA™ Miniprep Plus Kit. Six replicates of 25, 50, 100, and 200 µl of human whole blood were processed.

   

HSV-1 Viral DNA is Effectively Isolated from Plasma Using the Quick-DNA™ Miniprep Plus Kit. A dilution series of HSV-1 spiked into porcine plasma and extracted using the Quick-DNA™ Miniprep Plus Kit shows effective purification and subsequent qPCR amplification, even at a 20,000:1 dilution. The no template controls did not amplify even after 50 cycles.

Resuspend cultured cell or E. coli pellets using DNA Elution Buffer or an isotonic buffer (e.g. PBS): ˂ 1 x 106 cells in 100 µl 1-5 x 106 cells in 200 µl

Overnight Proteinase K digestions at 55ºC are possible without affecting the integrity of the DNA.


Biologicial Fluids & Cells

Solid Tissues

1. Add up to 200 µl sample to a microcentrifuge tube and add:

  • 200 µl BioFluid & Cell Buffer (Red)
  • 20 µl Proteinase K

2. Mix thoroughly or vortex 10-15 seconds and then incubate the tube at 55ºC for 10 minutes.

3. Add 1 volume Genomic Binding Buffer to the digested sample. Mix thoroughly or vortex 10-15 seconds.

1. To a tissue sample (≤ 25 mg) in a microcentrifuge tube, add a solution of:

  • 95 µl Water
  • 95 µl Solid Tissue Buffer (Blue)
  • 10 µl Proteinase K

2. Mix thoroughly or vortex 10-15 seconds and then incubate the tube at 55ºC for 1-3 hours or until tissue solubilizes. Mix thoroughly before proceeding.

3. Add 2 volumes Genomic Binding Buffer to the supernatant. Mix thoroughly or vortex 10-15 seconds.

  1. Transfer the mixture to a Zymo-Spin™ IIC-XL Column in a Collection Tube. Centrifuge at ≥ 12,000 x g for 1 minute. Discard the collection tube with the flow through.
  2. Add 400 µl DNA Pre-Wash Buffer to the spin column in a new Collection Tube. Centrifuge at ≥ 12,000 x g for 1 minute. Empty the collection tube.
  3. Add 700 µl g-DNA Wash Buffer to the spin column. Centrifuge at ≥ 12,000 x g for 1 minute. Empty the collection tube.
  4. Add 200 µl g-DNA Wash Buffer to the spin column. Centrifuge at ≥ 12,000 x g for 1 minute. Discard the collection tube with the flow through.
  5. Transfer the spin column to a clean microcentrifuge tube. Add ≥ 50 µl4 DNA Elution Buffer or water5 directly on the matrix. Incubate for 5 minutes at room temperature, then centrifuge at maximum speed for 1 minute to elute the DNA6. The eluted DNA can be used immediately for molecular based applications or stored ≤ -20°C for future use.
For specific notes and additional information, please see the product protocol PDF.
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“I was impressed with the yield. Usually spin column methods cause us to lose too much DNA, so we use other methods (e.g. salt extraction, phenol-chloroform), which are either laborious or yields impure samples. This method yielded an impressive quantity of DNA that seemed very clean.”
M.F. (University of Texas at Arlington)
“Improved yield than Bioline and Qiagen Kits.”
F.C. (Manchester Metropolitan University)
“I had good quality DNA, good yield out of the 200µl Human Blood, and the protocol was easy and fast.”
N.P. (University of Würzburg)
“I was very impressed with the purity of the DNA.”
C.V. (UCLA)
“Best product I have tested and used. Nothing to worry about. Yields and quality of genomic DNA is great and in no time.”
T.G. (Univeristy of Cologne)
"This kit is easy to use and provides a high quality yield."
M.B. (Syracuse University)
Click here to submit your review of the Quick-DNA™ Miniprep Plus Kit.