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Genomic DNA Clean & Concentrator™-10

Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
Unique spin column for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.

Product Size Catalog # Price Qty
Genomic DNA Clean & Concentrator™-10 25 Preps D4010
$91.00
Genomic DNA Clean & Concentrator™-10 100 Preps D4011
$298.00

About Genomic DNA Clean & Concentrator™-10

The Genomic DNA Clean & Concentrator™ (DCC™) is for the quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.


Format Spin Column (capped)
DNA Recovery Typically, up to 10 μg total DNA per column can be eluted with ≥10 μl water. Recovery of DNA ranges from 70 to 95%.
Equipment Microcentrifuge
DNA Size Limits Capable of purifying small DNA fragments >50 bp and large sized DNAs >200 kb.
Sample Sources DNA from impure preparations of genomic DNA (e.g., Proteinase K digestions), plasmid DNA (including BAC), viral DNA, and whole genome amplified (wga) DNA. Can also be used for the purification of lower molecular weight DNA (50 bp to 10 kb) from PCR, endonuclease digestion, post-RT cDNA synthesis, etc.
DNA Purity Ultra-pure (A(260/280) ≥ 1.8) high molecular weight DNA is eluted in water and is especially well suited for PCR, sequencing, endonuclease digestion, etc.
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS

Phage DNA Recovery. DNA (48.5 kb) is effectively recovered from 10-fold concentrations of starting material using the Genomic DCC™.
 

High molecular weight DNA is efficiently purified using the Genomic DCC™. Porcine gDNA (~35-50 kb), T4 phage DNA (170 kb), and DNA (48.5 kb) were purified (in duplicate) from input material using the Genomic DCC™. Eluted DNAs were analyzed in a 0.8% (w/v) TAE/agarose/EtBr gel (shown above). The size marker "M" is a 1 kb ladder (Zymo Research).

Step 1

In a 1.5 ml microcentrifuge tube, add 2-5 volumes of ChIP DNA Binding Buffer to each volume of DNA sample1 (see table below).  Mix thoroughly.

Application

DNA Binding Buffer  :  Sample

Example

Plasmid, genomic DNA (>2 kb)

2  :  1

200 µl  :  100 µl

PCR product, DNA fragment

5  :  1

500 µl  :  100 µl

Step 2

Transfer mixture to a provided Zymo-Spin™ IC-XL Column2 in a Collection Tube.

 

Step 3

Centrifuge for 30 seconds.  Discard the flow-through.

Step 4

Add 200 µl DNA Wash Buffer to the column.  Centrifuge for 1 minute.  Repeat the wash step.

Step 5

Add ≥ 10 µl DNA Elution Buffer3 or water4 directly to the column matrix and incubate at room temperature for one minute.  Transfer the column to a 1.5 ml microcentrifuge tube and centrifuge at for 30 seconds to elute the DNA.

Ultra-pure DNA is now ready for use.

For specific notes and additional information, please see the product protocol PDF.
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Featured Citations

The Genomic DNA Clean & Concentrator™-10 was used to clean gDNA extracted from human whole blood samples. Results showed high prevalence of asymptomatic infections and misdiagnosis of falciparum and vivax malaria in Tak parasite. This indicates higher than estimated transmission levels and the need for more sensitive malaria detection assays.
The Genomic DNA Clean & Concentrator™ was implemented for the purification of whole genome amplified DNA prior to generating DNA standards that are differentially methylated. This allowed accurate quantitation of methylation levels at the estrogen receptor alpha (ESR1) gene locus in the bovine endometrium

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