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ZR & ZR-96 Viral DNA/RNA Kits™

Quick co-purification of viral DNA/RNA from a wide range of sources (plasma, serum, etc.)
Zymo-Spin column & 96-well plate technology allows ultra-clean DNA and RNA to be eluted into minimal volumes.
Omits the use of organic denaturants and proteases.

Product Size Catalog # Price Qty
ZR Viral DNA/RNA Kit™ 25 Preps D7020
ZR Viral DNA/RNA Kit™ 100 Preps D7021
ZR-96 Viral DNA/RNA Kit™ 2x96 Preps D7022
ZR-96 Viral DNA/RNA Kit™ 4x96 Preps D7023

About ZR & ZR-96 Viral DNA/RNA Kits™

The ZR Viral DNA/RNA Kit™ provide for rapid isolation of high-quality viral nucleic acids from a wide range of biological sources. The kits can be used to successfully isolate viral DNA and RNA from cell-free body fluids (plasma, serum, etc.) as well as cellular suspensions at concentrations ≤ 1 x 105 cells/ml. The procedure employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA binding onto the column/plate matrix. The nucleic acids are washed then eluted with DNase/RNase-free Water. The eluted DNA and RNA are suitable for use in various subsequent procedures including RT/PCR.

Format Spin Column / 96-well spin plate
Equipment Microcentrifuge (column) / centrifuge with 96-well plate carrier (plate).
Sample Sources Plasma, serum, culture supernatants, animal cells and tissue.
Elution Volume ≥35 μl (column) / ≥10 μl (plate)
Storage Eluted DNA/RNA can be stored at ≤ -70°C
Sample Size ≤200 µl
Size Limit DNA/RNA fragments >50 bases.

The ZR Viral Kits™ from Zymo Research ensures high sensitivity viral detection compared to that of Supplier Q. Viral RNA was isolated from plasma samples using the ZR Viral RNA Kit™. Data are the mean (+/- SD) of triplicate RT-qPCR measurements.

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Featured Citations

Nucleic acids were isolated from viral particle enriched wastewater using the ZR Viral DNA/RNA Kit™ and then sequenced for metagenomics analysis on Illumina platforms. Based on alignments and a nonuniversal genetic code, results suggest the first described RNA viruses of ciliates.

“The ZR-96 viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”
S.B., Boehringer Ingelheim, Canada
Click here to submit your review of the ZR & ZR-96 Viral DNA/RNA Kits™.

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