About ZR & ZR-96 Viral DNA/RNA Kits™
The ZR Viral DNA/RNA Kit™ provide for rapid isolation of high-quality viral nucleic acids from a wide range of biological sources. The kits can be used to successfully isolate viral DNA and RNA from cell-free body fluids (plasma, serum, etc.) as well as cellular suspensions at concentrations ≤ 1 x 105 cells/ml. The procedure employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA binding onto the column/plate matrix. The nucleic acids are washed then eluted with DNase/RNase-free Water. The eluted DNA and RNA are suitable for use in various subsequent procedures including RT/PCR.
|Format||Spin Column / 96-well spin plate|
|Equipment||Microcentrifuge (column) / centrifuge with 96-well plate carrier (plate).|
|Sample Sources||Plasma, serum, culture supernatants, animal cells and tissue.|
|Elution Volume||≥35 μl (column) / ≥10 μl (plate)|
|Storage||Eluted DNA/RNA can be stored at ≤ -70°C|
|Sample Size||≤200 µl|
|Size Limit||DNA/RNA fragments >50 bases.|
The ZR Viral Kits™ from Zymo Research ensures high sensitivity viral detection compared to that of Supplier Q. Viral RNA was isolated from plasma samples using the ZR Viral RNA Kit™. Data are the mean (+/- SD) of triplicate RT-qPCR measurements.
Nucleic acids were isolated from viral particle enriched wastewater using the ZR Viral DNA/RNA Kit™ and then sequenced for metagenomics analysis on Illumina platforms. Based on alignments and a nonuniversal genetic code, results suggest the first described RNA viruses of ciliates.
“The ZR-96 viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”
S.B., Boehringer Ingelheim, Canada