About ZymoPURE™ Plasmid Gigaprep Kit
ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE™ Plasmid Gigaprep kit features a spin column-based method for the purification of up to 10 mg of high-quality plasmid DNA in less than 50 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
As an added convenience, the ZymoPURE™ Plasmid Gigaprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Also, bottle top filters are included for rapid clearing of the lysate.
|Processing Time||50 min|
|DNA Purity||Eluted DNA is ultrapure and well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications.|
|Plasmid DNA Size||Up to 25 kb|
|Plasmid DNA Yield||Up to 10 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
|Recovery Volume||≥ 2 ml of ZymoPURE™ Elution Buffer or DNase free water|
|Equipment Needed||Vacuum/vacuum manifold and swinging bucket centrifuge.|
|Recommended Sample Volume||2.5 L E. coli Culture|