Zyppy™ Plasmid Miniprep Kit

The fastest, easiest miniprep available for purifying transfection quality plasmid DNA.
Pellet-free procedure omits conventional cell pelleting and resuspension steps.
DNA quality appropriate for cloning, sequencing, and transfection.

Product Size Catalog # Price Qty
Zyppy™ Plasmid Miniprep Kit 50 Preps D4036
$55.00
Zyppy™ Plasmid Miniprep Kit 100 Preps D4019
$100.00
Zyppy™ Plasmid Miniprep Kit 400 Preps D4020
$336.00
Zyppy™ Plasmid Miniprep Kit 800 Preps D4037
$612.00

About Zyppy™ Plasmid Miniprep Kit

The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using the provided Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.


Format Spin Column
Processing Time 8 min
DNA Purity Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) index is ≥1.8.
Elution Volume ≥ 30 µl
Plasmid DNA Size Up to 25 kb
Plasmid DNA Yield Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
Procedure Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold.

 

DNA yield from Zyppy™ Plasmid Miniprep Kit and a kit from Supplier A. Plasmid DNA (pGEM®) was digested with EcoRI prior to agarose gel electrophoresis. Performed in triplicate. M, ZR 1 kb DNA Marker (Zymo Research)

Step 1

Add 600 µl of bacterial culture grown in LB medium to a 1.5 ml microcentrifuge tube.

The Zyppy™ Plasmid Miniprep Kit may also be used with the classical centrifuge-based procedure for processing up to 3 ml of bacterial culture.  The procedure should be modified as follows:  1A) Centrifuge 1.5 ml of bacterial culture for 30 seconds at maximum speed.  1B) Discard the supernatant.  1C) Repeat steps 1A and 1B as needed.  1D) Add 600 μl of TE or water to the bacterial cell pellet and resuspend completely. 

Step 2

Add 100 µl of 7X Lysis Buffer (Blue)1 and mix by inverting the tube 4-6 times.  Proceed to step 3 within 2 minutes.

After addition of 7X Lysis Buffer the solution should change from opaque to clear blue, indicating complete lysis. 

Step 3
Add 350 µl of cold Neutralization Buffer (Yellow) and mix thoroughly.  The sample will turn yellow when the neutralization is complete and a yellowish precipitate will form.  Invert the sample an additional 2-3 times to ensure complete neutralization.
Step 4

Centrifuge at 11,000 – 16,000 x g for 2-4 minutes.

Step 5

Transfer the supernatant (~900 µl) into the provided Zymo-Spin™ IIN column.  Avoid disturbing the cell debris pellet.

Step 6

Place the column into a Collection Tube and centrifuge for 15 seconds.

Step 7

Discard the flow-through and place the column back into the same Collection Tube.

Step 8

Add 200 µl of Endo-Wash Buffer to the column.  Centrifuge for 30 seconds.  It is not necessary to empty the collection tube.

Step 9

Add 400 µl of Zyppy™ Wash Buffer to the column.  Centrifuge for 1 minute.

Step 10

Transfer the column into a clean 1.5 ml microcentrifuge tube then add 30 µl of Zyppy™ Elution Buffer2 directly to the column matrix and let stand for one minute at room temperature.

Step 11

Centrifuge for 30 seconds to elute the plasmid DNA.

For specific notes and additional information, please see the product protocol PDF.
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Featured Citations

The Zyppy™ Plasmid MiniPrep was utilized to isolate high quality plasmids for phylogenetic analysis to study methanogen diversity in animal hosts. The data was used to demonstrate the potential to develop markers to track sources of fecal pollution.
Zyppy™ Plasmid MiniPrep kit used in extraction of pure plasmid DNA in a university research-based laboratory course, stimulating student interest in bioinformatics and molecular biology while conducting original research.
High quality plasmid DNA was isolated from E. coli using the Zyppy™ Plasmid Miniprep Kit for the purpose of bioengineering E. coli to generate significantly higher titers of resveratrol (~60% increase). This innovation could potentially lead to the development of an alternative supply of resveratrol for the pharmaceutical, nutraceutical and food industry.

“Performed side-by-side preparations of single culture split into two, using the Zyppy and a leading competitor's miniprep kit. The Zyppy kit saved me ~25 minutes and returned plasmid of better 260/280.”

Greg G. (North Carolina State University)

“Fast, efficient purification, good with elution of concentrated DNA thanks to a different spin column design, fewer buffers and fewer steps than in other minprep protocols, all buffers prepared and ready to use straight out of the package, good price. Please don't change anything, the kit works great.”

Sebastian K (Lund University)

“Simple procedure was easy to follow, high yield. Blue/yellow color visualization. In spite of all the advantages of above, it is inexpensive!!!”

Hyejin Y. (Washington University School of Medicine.)

“It's so FAST!  And it works!! I can't think of a way to improve this product.  My big problem with it is that it is SO fast that I don't have time to label my tubes!”

Anita F. (Fairfield University)

“The kit gives a very good yield of plasmid DNA of up to 12 kb in size. I like the fact that procedure can be modified  for purification of plasmid DNA from up to 3 ml of bacterial culture.”

Vladimir B (Touro University Nevada)

“The quality of the DNA is very good. Sequencing reads are generally beautiful.”

Magdalena B. (University of Massachusetts, Amherst)

“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min.  Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”

Rebecca N.

“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard).  The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”

Richard F.

"The results gave what they promised!  Clean DNA.">/em<

Pamela R (UT Medicine San Antonio)
The speed of sample processing obviously. I did 18 samples in one run, it was very easy to perform physically and psychologically :). I like that your plastic ware is much better designed than Qiagen's plastic ware. For example it seems to be taller and holds the wash liquids in much better. I also tested a Qiagen kit in parallel with yours and the plasmid yield was significantly better. For routine plasmid preps that we do in our lab, its works great. The price is great too.
Maria Volnoukhin (Simon Fraser University)
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