About Mix & Go Competent Cells - Strain Zymo 5α
The Mix & Go E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go competent cells are highly efficient (> 108 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Premade Mix & Go competent cells strain Zymo 5α are supplied as a pack of 10 convenient single use aliquots, as a 96-tube rack with removable 8-tube strips, or as a 96-well PCR plate for your high-throughput transformation needs.
|Format||Either as a pack of ten single use aliquots (100 µl / tube), as a 96-tube rack with removable 8-tube strips (50 µl / tube), or as a 96-well PCR plate (50 µl / well)|
|Genotype||F-φ80lacZ∆M15 ∆(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1, λ-|
|Additional Information||Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.|
The mechanisms of electrotransfection of mammalian cells were determined by observing the interaction of plasmid DNA (pDNA) with the cell membrane and internalization of the pDNA into the cell. The plasmid used in the study was amplified using Mix & Go E. coli Competent Cells, strain Zymo 5α, from Zymo Research, and labeled with a fluorescence dye to visualize pDNA binding and internalization. The authors found that efficient electrotransfection is dependent on the presence of divalent cations and endocytic pathways that can transport the pDNA across the membrane.
In the process of developing a protocol to facilitate real-time imaging of apoptotic cell membrane changes in live cells, researchers used the Mix & Go Z-competent E.coli strain Zymo 5α from Zymo Research for fast, high-efficiency transformations to express annexin B12 modified with fluorophores that fluoresce when bound to phosphatidylserine-containing membranes.