DNA Hydroxymethylation

DNA Hydroxymethylation Overview

Over the past several years, many laboratories have been intensely investigating DNA hydroxymethylation, yet its biological role is still not completely understood. 5-hydroxymethylcytosine (5-hmC) is found in many organisms and is present at high levels in human and mouse neurological tissue. This modified base, often considered the “sixth base”, is speculated to be an intermediate in oxidative DNA demethylation pathways, but could itself constitute a new layer of epigenetic regulation. 5-hmC has gained attention in recent years as a potential epigenetic factor in many fields, including stem cell renewal, cancer development and progression, and neurological disorders. Furthermore, many standard methods used for detecting methylated DNA, such as bisulfite conversion, cannot distinguish between 5-mC and 5-hmC, so therefore it is critical to accompany DNA methylation studies with experiments that can differentiate between these two modifications.

Zymo Research was one of the pioneers to provide products and services to investigate 5-hydroxymethylcytosine (5-hmC). Today, Zymo Research remains the industry leader for investigating this novel epigenetic modification. A complete line of products is available to aid researchers interested in investigating DNA hydroxymethylation, including hydroxymethylated DNA standards, enzymes, kits, and services for both region-specific and genome-wide determination of 5-hmC levels.


DNA Hydroxymethylation Technologies

Hydroxymethylated DNA Standards

Accurately assess the levels of 5-hmC in DNA samples using Zymo Research’s hydroxymethylated DNA controls and standards.  (Learn More)


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5-hmC Glucosyltransferase

Easily distinguish 5-hmC in DNA from 5-mC by completely glucosylating all 5-hydroxymethylated cytosines for easy enrichment with JBP-1 or to render specific DNA sequences resistant to restriction enzyme digestion.  (Learn More)


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5-hmC ELISA

Rapid, high-throughput adaptable assay to quickly, specifically, and accurately detect and quantify global 5-hydroxymethylcytosine levels in DNA.  (Learn More)


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5-hmC Antibodies & Enrichment

Immunodetection and enrichment of 5-hmC modified DNA with either our 5-hmC antibody or our innovative JBP-1-based assay.  (Learn More)


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Region-Specific 5-hmC Analysis

Zymo Research offers robust bisulfite-free assays for locus-specific detection of 5-hmC levels in DNA.  (Learn More)


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Global 5-hmC Quantification

The extent of DNA hydroxymethylation can be accurately determined on a global scale by mass spectrometry or sensitive chromatography methods following digestion of the DNA to individual nucleoside or nucleotide components.  (Learn More)


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