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5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set

Control DNA for 5-methylcytosine and 5-hydroxymethylcytosine quantitation applications (i.e. - mass spectrometry, HPLC, TLC, etc.)
Substrate for studies involving 5-hydroxymethylcytosine interacting proteins

Product Size Catalog # Price Qty
5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set 1 set D5405

About 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set

The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three DNA standards that are linear dsDNA, 897 bp, and have the same sequence (see sequence info & figure). The only difference is that each contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines (see figure). Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.

Concentration 50 ng/µl in storage buffer (10 mM Tris-HCl; 0.1 mM EDTA; pH 8.0). All standards are supplied in 40 µl volume.
1 set Includes 3 tubes each containing 2 µg of 100% unmodified cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine DNA standard.
Storage Store reagents at -20 °C for up to 12 months. Prolonged storage should be ≤ -70 °C.

Figure. 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set
All three standards are 897 bp with cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), or 5-hydroxymethylcytosine (lane 3, 5hmC). 1kb DNA ladder (Zymo Research).

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The DNA standard set served as a control to verify the specificity of anti-5-mC and anti-5-hmC antibodies by dot blots. These antibodies were then used for 5-hmC and 5-mC-specific immunostaining of metaphase chromosomes.

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