About 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set
The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three DNA standards that are linear dsDNA, 897 bp, and have the same sequence (see sequence info & figure). The only difference is that each contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines (see figure). Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.
Specifications
| Concentration | 50 ng/µl in storage buffer (10 mM Tris-HCl; 0.1 mM EDTA; pH 8.0). All standards are supplied in 40 µl volume. |
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| 1 set | Includes 3 tubes each containing 2 µg of 100% unmodified cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine DNA standard. |
| Storage | Store reagents at -20 °C for up to 12 months. Prolonged storage should be ≤ -70 °C. |
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Figure. 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set |
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