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Human Matched DNA Set

Matched set of human genomic DNA from brain and spleen originating from a single individual
Precisely quantified levels of 5-methylcytosine & 5-hydroxymethylcytosine via LC / MS
Useful control for detection methods of 5-methylcytosine or 5-hydroxymethylcytosine

Product Size Catalog # Price Qty
Human Matched DNA Set 1 set D5018
$536.00

About Human Matched DNA Set

The Human Matched DNA Set is a set of organ specific human genomic DNAs originating from a single individual. This set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine as both modified cytosines are present at physiologically relevant levels and loci. The levels of 5-hydroxymethylcytosine within the Human Matched DNA Set have been quantified and are listed below.

 

LC/MS Quantification
500ng of brain and spleen gDNA from Human Matched DNA Set were digested with DNA Degradase Plus for 1 hour. Processed DNA was subsequently run on LC / MS for precise global quantification of 5mC and 5hmC percentages.

Match Set Human Genomic DNA 1 µg of Brain and Spleen gDNA were resolved in a 0.8% agarose gel. “M” denotes 1 kb DNA ladder (Zymo Research Corp.)

Note: Some degradation of genomic DNA originating from organs may be unavoidable due to processing time of tissue post-mortem.

**For human genomic DNA with extremely low levels (<0.03%) of 5-hydroxymethylcytosine, please refer to Human HCT116 DKO Non-methylated DNA (Zymo Research Corp., Cat. No. D5014-1).


Concentration 250 ng/µl in modified TE buffer (10mM Tris-HCl; 0.1mM EDTA; pH 8.0)
Source Human male
Storage ≤ -20°C

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