Mouse 5-hmC & 5-mC DNA Set

Precisely quantified levels of 5-methylcytosine and 5-hydroxymethylcytosine verified via mass spectrometry (LC/MS)
Useful control for detection methods of 5-methylcytosine or 5-hydroxymethylcytosine

Product Name Size Catalog # Price Qty
Mouse 5-hmC & 5-mC DNA Set n/a D5019
$454.00

About Mouse 5-hmC & 5-mC DNA Set

The Mouse 5-hmC & 5-mC DNA Set is a set of organ specific mouse genomic DNAs isolated from a pool of 8-10 week old Swiss Webster mice. This genomic DNA set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine as both modified cytosines are present at physiologically relevant levels and loci. The levels of 5-methylcytosine and 5-hydroxymethylcytosine within the Mouse 5-hmC & 5-mC DNA Set have been precisely quantified by mass spectrometry (LC/MS) and been validated to be consistent by ELISA. Percentages of each modified cytosine are listed below.

Specifications

Concentration 250 ng/μl in modified TE buffer (10mM Tris-HCl; 0.1mM EDTA; pH 8.0)
Source Swiss Webster mice, 8-10 weeks old.
Storage ≤ -20°C

Precise Quantification of 5mC and 5hmC via LC/MS
5-mC and 5-hmC levels within genomic DNA isolated from mouse organs were quantified by LC/MS. 5-mC and 5-hmC levels are expressed as percentage of total cytosines.

Mouse 5-hmC & 5-mC DNA Set ELISA Assay
Anti-5-methylcytosine or anti-5-hydroxymethylcytosine antibodies were confidently able to reproduce trends in levels of 5-methylcytosine and 5-hydroxymethylcytosines quantified by mass spectrometry.