About Mouse 5hmC & 5mC DNA Set
The Mouse 5-hmC & 5-mC DNA Set is a set of organ specific mouse genomic DNAs isolated from a pool of 8-10 week old Swiss Webster mice. This genomic DNA set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine as both modified cytosines are present at physiologically relevant levels and loci. The levels of 5-methylcytosine and 5-hydroxymethylcytosine within the Mouse 5-hmC & 5-mC DNA Set have been precisely quantified by mass spectrometry (LC/MS) and been validated to be consistent by ELISA. Percentages of each modified cytosine are listed below.
|Concentration||250 ng/µl in modified TE buffer (10mM Tris-HCl; 0.1mM EDTA; pH 8.0)|
|Source||Swiss Webster mice, 8-10 weeks old.|
Precise Quantification of 5mC and 5hmC via LC/MS
Mouse 5-hmC & 5-mC DNA Set ELISA Assay