About EZ-96 DNA Methylation™ Kit
The EZ-96 DNA Methylation™ Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product's innovative in-column desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. Note: Cat. No. D5004 is recommended by Illumina for its GoldenGate® and Infinium® Arrays.
|Learn More About Bisulfite Conversion||Click to see which kit is right for you|
|DNA Recovery||> 80%|
|Processing Time||12 - 16 hrs|
|Equipment||Centrifuge with microplate carriers|
|Binding Capacity||5 µg/well|
|Elution Volume||Deep-well: ≥ 15 µl, Shallow-well: ≥ 30 µl|
|Conversion Efficiency||> 99%|
|Input||Plasmid, genomic, and endonuclease digested DNAs.|
Conversion of non-methylated cytosine. A 459 bp PCR product amplified from either DNA converted with the EZ DNA Methylation™ Kit (lane 1) or from untreated DNA (lane 2) was digested with EcoR I. The cytosine at the EcoR I site was converted to uracil during treatment, preventing cleavage of the DNA by the endonuclease.
Researchers from Barcelona recently used the EZ-96 DNA Methylation Kit™ to successfully validate the new MethylationEPIC BeadChIP (Infinium 850K array, Illumina) with FFPE samples. The results validate the 850K array as a highly reliable genomic platform for the study of DNA methylation patterns and levels in the human genome. The data also represents an extremely valuable tool to decipher how DNA methylation changes in unexplored territories, such as enhancer sequences, contribute to cell homeostasis and human diseases.
In a genome-wide study investigating DNA methylation levels in acute myeloid leukemia cells, researchers used the EZ DNA MethylationTM Kit to bisulfite convert genomic DNA obtained from clinical samples. PCR amplification followed by bisulfite sequencing of the converted DNA helped to show that patients diagnosed with acute myeloid leukemia have distinct methylomes in their cancer cells compared to normal cells.