About EZ-96 DNA Methylation-Direct™ Kit
The EZ-96 DNA Methylation-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation-Gold™ kits. These products feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold™ kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
Specifications
| Format | 96-Well |
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| DNA Recovery | > 80% |
| Processing Time | 4 hrs |
| Equipment | Centrifuge with microplate carrier |
| Binding Capacity | 5 µg/well |
| Elution Volume | Shallow-well: ≥ 30 µl, Deep-well: ≥ 15 µl |
| Conversion Efficiency | > 99.5% |
| Input | DNA, cells, blood, tissue, FFPE |
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EZ DNA Methylation-Direct™ Kit bisulfite chemistry significantly improves C to U conversion kinetics. DNA was converted using either EZ DNA Methylation-Direct™ or conventional bisulfite chemistries. Recovered DNA was amplified by PCR, then cloned. Sequences from individual clones were analyzed and quantitated. These data show that EZ DNA Methylation-Direct™ bisulfite chemistry improves the rate and extent (> 99.8%) of C to U conversion of DNA as compared to conventional bisulfite chemistry.
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