About EZ DNA Methylation™ Kit
The EZ DNA Methylation™ Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product's innovative in-column desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. This kit is validated for use with Illumina's GoldenGate® and Infinium® Assays.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | > 80% |
| Processing Time | 12 - 16 hrs |
| Equipment | Microcentrifuge |
| Binding Capacity | 5 µg/prep |
| Elution Volume | ≥ 10 µl |
| Conversion Efficiency | > 99% |
| Input | Plasmid, genomic, and endonuclease digested DNAs. |
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Conversion of non-methylated cytosine. A 459 bp PCR product amplified from either DNA converted with the EZ DNA Methylation™ Kit (lane 1) or from untreated DNA (lane 2) was digested with EcoR I. The cytosine at the EcoR I site was converted to uracil during treatment, preventing cleavage of the DNA by the endonuclease.
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